Bcl-xL Antibody (HL2038) - Azide and BSA Free

Western Blot: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemistry-Paraffin: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Bcl-XL antibody [HL2038] detects Bcl-XL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat intestine. Bcl-XL stained by Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Bcl-xL Antibody (HL2038) - Azide and BSA Free [NBP3-25383] - Bcl-XL antibody [HL2038] detects Bcl-XL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Bcl-XL stained by Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Bcl-XL antibody [HL2038] detects Bcl-XL protein by immunofluorescent analysis.Sample: MDCK cells were fixed in ice-cold MeOH for 5 min.Green: Bcl-XL stained by Bcl-XL antibody [HL2038] (NBP3-25383) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Ca, Fe
• Applications: WB, IHC
• Clone: HL2038
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Bcl-xL Antibody (HL2038) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Bcl-xL Antibody (HL2038) - Azide and BSA Free (NBP3-25383) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Bcl-xL
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BCL2L1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Bcl-xL Antibody (HL2038) - Azide and BSA Free

• Apoptosis regulator Bcl-X
• bcl2-L-1
• BCL2-like 1
• bcl-2-like protein 1
• BCLXBCL2LBcl-X
• BclxL
• Bcl-xL
• BCL-XL/S
• BCLXS
• bcl-xS
• DKFZp781P2092

Background

Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL2L1