Bcl-2 Antibody - BSA Free

Western Blot: Bcl-2 Antibody [NBP2-30108] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), Bcl-2 from Company B, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] - Figure 7 Immunofluorescence Validation of Bcl-2 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling Bcl-2 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP2-30108] - Immunohistochemistry Validation of Bcl-2 in Human Kidney. Immunohistochemical analysis of paraffin-embedded Human Kidney using anti-Bcl-2 antibody (NBP2-30108) at 2ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: Bcl-2 Antibody [NBP2-30108] - Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human Daudi cellsLane B: Mouse A-20 cells

Western Blot: Bcl-2 Antibody [NBP2-30108] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: Bcl-2 Antibody - BSA Free [NBP2-30108] - Figure 6 Immunohistochemistry Validation of Bcl-2 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using anti-Bcl-2 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] - Validation of Bcl-2 in Human Kidney Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Kidney Cells labeling Bcl-2 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bcl-2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 15 amino acids near the N terminus of human Bcl-2. The immunogen is located within the first 50 amino acids of Bcl-2. Amino Acid Squence: KYIHYKLSQRGYEWD
• Specificity: Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD). Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD). Rat Bcl-2 has one isoform (236aa, 27kD). NBP2-30108 can detect all isoforms of human, mouse and rat.
• Predicted Species: Rat (100%), Chicken (93%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 10ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2ug/ml
  • Western Blot 1-2ug/ml
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Bcl-2 Antibody - BSA Free

• apoptosis regulator Bcl-2
• B-cell CLL/lymphoma 2
• Bcl2
• Bcl-2

Background

cl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL2
• Uniprot:
  • AAH27258(Human)