BAK Antibody - BSA Free

Western Blot: BAK Antibody [NBP1-77152] - Analysis of Bak in L1210 cell lysates with Bak antibody using NBP1-77152 at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: BAK Antibody - BSA Free [NBP1-77152] - Immunocytochemistry of BAK in L1210 cells with BAK antibody at 2 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BAK Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BAK Antibody - BSA Free (NBP1-77152) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-BAK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 13 amino acids near the amino-terminus of human Bak. The immunogen is located within amino acids 30 - 80 of Bak. Amino Acid Squence: QEQEAEGVAAPAD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAK1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  BAK Peptide (NBP1-77152PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for BAK Antibody - BSA Free

• Apoptosis regulator BAK
• BAK
• BAK1
• BAK-LIKE
• BAKMGC3887
• BCL2-antagonist/killer 1
• BCL2L7
• bcl2-L-7
• BCL2L7bcl-2 homologous antagonist/killer
• BCL2-like 7 protein
• Bcl-2-like protein 7
• CDN1
• MGC117255
• pro-apoptotic protein BAK

Background

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bak, a pro-apoptotic Bcl-2 family member, is an oligomeric protein that localizes to the mitochondria. It is thought to share significant functional homology with Bax, another pro-apoptotic Bcl-2 family member, as disruption of bak or bax has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking bak and bax show decreased apoptotic capability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAK Antibody (NBP1-77152)(1)

Publication using NBP1-77152

• Marwarha G, Rosand O, Slagsvold KH, Hoydal MA GSK3 beta Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia International journal of molecular sciences 2022-08-19 [PMID: 36012628] (S-ELISA, Human)

Bioinformatics

• Gene Symbol: BAK1
• Uniprot:
  • Q16611(Human)