BAK Antibody

Images

 
Western Blot: BAK Antibody [NBP1-77152] - Analysis of Bak in L1210 cell lysates with Bak antibody using NBP1-77152 at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry: BAK Antibody [NBP1-77152] - Staining of L1210 cells at 2 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BAK Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 13 amino acids near the amino-terminus of human Bak. The immunogen is located within amino acids 30 - 80 of Bak.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAK1
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunocytochemistry
Application Notes
Bak antibody can be used for detection of Bak by Western blot at 1 to 2 mg/ml.
Control Peptide
BAK Peptide (NBP1-77152PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for BAK Antibody

  • Apoptosis regulator BAK
  • BAK
  • BAK1
  • BAK-LIKE
  • BAKMGC3887
  • BCL2-antagonist/killer 1
  • BCL2L7
  • bcl2-L-7
  • BCL2L7bcl-2 homologous antagonist/killer
  • BCL2-like 7 protein
  • Bcl-2-like protein 7
  • CDN1
  • MGC117255
  • pro-apoptotic protein BAK

Background

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bak, a pro-apoptotic Bcl-2 family member, is an oligomeric protein that localizes to the mitochondria. It is thought to share significant functional homology with Bax, another pro-apoptotic Bcl-2 family member, as disruption of bak or bax has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking bak and bax show decreased apoptotic capability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BAK Antibody (NBP1-77152)

Discover related pathways, diseases and genes to BAK Antibody (NBP1-77152). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for BAK Antibody (NBP1-77152)

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Blogs on BAK.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

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Bioinformatics

Gene Symbol BAK1
Entrez
Uniprot