BAK Antibody (8D1) - BSA Free

Immunoprecipitation: BAK Antibody (8D1) [NBP3-26482] - Immunoprecipitating BAK in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26482 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26482 (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).

Immunohistochemistry: BAK Antibody (8D1) [NBP3-26482] - Image of BAK Antibody (8D1) diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry: BAK Antibody (8D1) [NBP3-26482] - Image of BAK Antibody (8D1) diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: BAK Antibody (8D1) [NBP3-26482] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Colo320 whole cell lysate. All lanes: BAK Antibody at 0.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 24, 17 KDa Observed band size: 24 KDa

Flow Cytometry: BAK Antibody (8D1) [NBP3-26482] - Overlay histogram showing Hela cells stained with BAK Antibody (8D1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IP
• Clone: 8D1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BAK Antibody (8D1) - BSA Free Summary

• Description: Novus Biologicals Rabbit BAK Antibody (8D1) - BSA Free (NBP3-26482) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human BAK [UniProt Q16611]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BAK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for BAK Antibody (8D1) - BSA Free

• Apoptosis regulator BAK
• BAK
• BAK1
• BAK-LIKE
• BAKMGC3887
• BCL2-antagonist/killer 1
• BCL2L7
• bcl2-L-7
• BCL2L7bcl-2 homologous antagonist/killer
• BCL2-like 7 protein
• Bcl-2-like protein 7
• CDN1
• MGC117255
• pro-apoptotic protein BAK

Background

Bak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAK1