BAG2 Antibody (NBP1-59087)

Images

 
Western Blot: BAG2 Antibody [NBP1-59087] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml
Immunohistochemistry-Paraffin: BAG2 Antibody [NBP1-59087] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: BAG2 Antibody [NBP1-59087] - Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human.
Western Blot: BAG2 Antibody [NBP1-59087] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BAG2 Antibody Summary

Immunogen
Synthetic peptides corresponding to BAG2(BCL2-associated athanogene 2) The peptide sequence was selected from the C terminal of BAG2. Peptide sequence VDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSK.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAG2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against BAG2 and was validated on Western Blot and immunohistochemistry-paraffin
Publications
Read Publication using
NBP1-59087 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26729625)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for BAG2 Antibody

  • BAG family molecular chaperone regulator 2
  • BAG-2
  • BAG-family molecular chaperone regulator-2
  • Bcl-2-associated athanogene 2
  • BCL2-associated athanogene 2
  • dJ417I1.2 (BAG-family molecular chaperone regulator 2)
  • dJ417I1.2
  • KIAA0576
  • MGC149462

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAG2 Antibody (NBP1-59087)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BAG2
Uniprot