Western Blot: MAPKAPK2 Antibody (2B3) [H00009261-M01] - MAPKAPK2 monoclonal antibody (M01), clone 2B3 Analysis of MAPKAPK2 expression in K-562.
Immunocytochemistry/ Immunofluorescence: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of protein-protein interactions between AKT1 and MAPKAPK2. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and ...read more
Immunoprecipitation: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of MAPKAPK2 transfected lysate using anti-MAPKAPK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK2 MaxPab rabbit ...read more
ELISA: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Detection limit for recombinant GST tagged MAPKAPK2 is approximately 0.1ng/ml as a capture antibody.
MAPKAPK2 (NP_116584, 302 a.a. ~ 400 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARALEAAALAH
MAPKAPK2 - mitogen-activated protein kinase-activated protein kinase 2
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Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.In Simple Western only 10-15 uL of the recommended dilution is used per data point. Simple Western reported by an internal validation. Separated by Size, antibody dilution of 1:50, detects a band at 50 kDa.
Read 1 Review rated 4 using H00009261-M01 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
In 1x PBS, pH 7.4
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for MAPKAPK2 Antibody (2B3)
MAP kinase-activated protein kinase 2
MAPK-activated protein kinase 2
MAPKAP kinase 2
mitogen-activated protein kinase-activated protein kinase 2
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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