MAPKAPK2 Antibody (2B3)

Images

 
Western Blot: MAPKAPK2 Antibody (2B3) [H00009261-M01] - MAPKAPK2 monoclonal antibody (M01), clone 2B3 Analysis of MAPKAPK2 expression in K-562.
Immunocytochemistry/ Immunofluorescence: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of protein-protein interactions between AKT1 and MAPKAPK2. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and ...read more
Immunoprecipitation: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of MAPKAPK2 transfected lysate using anti-MAPKAPK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK2 MaxPab rabbit ...read more
ELISA: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Detection limit for recombinant GST tagged MAPKAPK2 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, PLA
Clone
2B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MAPKAPK2 Antibody (2B3) Summary

Immunogen
MAPKAPK2 (NP_116584, 302 a.a. ~ 401 a.a) partial recombinant protein with GST tag.IRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARALEAAALAH
Specificity
MAPKAPK2 - mitogen-activated protein kinase-activated protein kinase 2
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAPKAPK2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Proximity Ligation Assay
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 4
using
H00009261-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAPKAPK2 Antibody (2B3)

  • EC 2.7.11
  • EC 2.7.11.1
  • MAP kinase-activated protein kinase 2
  • MAPK-activated protein kinase 2
  • MAPKAP kinase 2
  • MAPKAPK2
  • MAPKAPK-2
  • mitogen-activated protein kinase-activated protein kinase 2
  • MK2
  • Rps6kc1

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for MAPKAPK2 Antibody (H00009261-M01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00009261-M01:
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Images Ratings Applications Species Date Details
Western Blot MAPKAPK2 H00009261-M01
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reviewed by:
Anonymous
WB Human 10/24/2018
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Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman
LotD6201-2B3

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAPKAPK2 Antibody (H00009261-M01) (0)

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Anonymous
10/24/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAPKAPK2
Entrez
OMIM
Uniprot