Bag-1 Antibody - BSA Free

Western Blot: Bag-1 Antibody [NB100-56082] - Analysis of BAG-1 in normal brain tissue lysates.

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082] - Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using this antibody at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-eosin counterstain.

Western Blot: Bag-1 Antibody [NB100-56082] - BAG-1 levels in human neuroblastoma. Image from verified customer review.

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082] - Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using NB100-56082 at 1:2000. A1 is a higher magnification of A. Differential Bag-1 expression levels are seen in the hypothalmic nuclei. The variation in expression levels from lack of Bag-1 staining to strong staining may be linked to differential responses of the hypothalmic nuclei to ischemic stress. Hematoxylin-eosin counterstain.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Bag-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Bag-1 Antibody - BSA Free (NB100-56082) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-Bag-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acids 26-45 (NERYDLLVTPQQGNSEPVVQD) of mouse BAG-1 was used as immunogen; GenBank no. AAH03722.1. This sequence is 100% conserved in mouse BAG-1L; GenBank no. NP_033866.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAG1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Control:

  Bag-1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for Bag-1 Antibody - BSA Free

• BAG family molecular chaperone regulator 1
• Bag1
• Bag-1
• Bcl-2 associating athanogene-1 protein
• Bcl-2-associated athanogene 1
• BCL2-associated athanogene
• Bcl-2-binding protein
• glucocortoid receptor-associated protein RAP46,46-KD
• HAP
• RAP46

Background

The BAG (Bcl-2-associated anthanogene) proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S , BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (Kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). NB100-56082 recognizes both BAG-1 and BAG-1 isoforms which contain the peptide immunogen sequence, including BAG-1L. Mouse BAG-1 is a 219 amino acid (aa) protein (GenBank no. AAH03722.1) and mouse BAG-1L is a 355 aa protein (AAH03722.1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bag-1 Antibody (NB100-56082)(1)

Publication using NB100-56082

• Aust Stefanie, Pils Sophie, Polterauer Stefan et al. Expression of Bcl-2 and the antiapoptotic BAG family proteins in ovarian cancer. Appl Immunohistochem Mol Morphol. 2013-12-01 [PMID: 23455184] (Human)

Bioinformatics

• Gene Symbol: BAG1
• Entrez:
  • 573(Human)
  • 12017(Mouse)
  • 297994(Rat)