CDT2 Antibody

Images

 
Western Blot: CDT2 Antibody [NB100-40840] - Detection of Human DTL/CDT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: CDT2 Antibody [NB100-40840] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: CDT2 Antibody [NB100-40840] - Detection of Human DTL/CDT2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; ...read more
Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:250. Detection: DAB staining using ...read more
Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - Human colon carcinoma (left) and mouse hybridoma tumor (right). Antibody used at a dilution of 1:1,000.
Immunohistochemistry: CDT2 Antibody [NB100-40840] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

CDT2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 680 and the C-terminus (residue 730) of human Denticleless Homolog (Chromatin Licensing and DNA Replicating Factor 2) using the numbering given in entry NP_057532.1 (GeneID 51514)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DTL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Positive Control
CDT2 Lysate (NBL1-10031)
Reviewed Applications
Read 1 Review rated 4
using
NB100-40840 in the following applications:

Publications
Read Publications using
NB100-40840 in the following applications:

  • IP
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDT2 Antibody

  • CDT2RA-regulated nuclear matrix-associated protein
  • CDW1
  • DCAF2DDB1 and CUL4 associated factor 2
  • DDB1- and CUL4-associated factor 2
  • denticleless homolog (Drosophila)
  • L2DTLRA regulated nuclear matrix associated protein
  • Lethal(2) denticleless protein homolog
  • RAMPdenticleless protein homolog
  • Retinoic acid-regulated nuclear matrix-associated protein

Background

DTL promotes genomic stability through two distinct mechanisms. First, it is an essential component of the CUL4-DDB1 complex that controls CDT1 levels, thereby preventing rereplication. Second, it is required for the early G2/M checkpoint.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDT2 Antibody (NB100-40840)(4)

Review for CDT2 Antibody (NB100-40840) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-40840:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23478441
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23478441

Details

Detection NotesSee PMID 23478441

Comments

CommentsPublished in PMID: 23478441

Product General Protocols

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FAQs for CDT2 Antibody (NB100-40840) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
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4
1
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2
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1
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Anonymous
12/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol DTL
Entrez
Uniprot