Endothelial Lipase Antibody

Images

 
Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)
Immunocytochemistry/ Immunofluorescence: Endothelial Lipase Antibody [NB400-111] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Western Blot: Endothelial Lipase Antibody [NB400-111] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY LIPG cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were ...read more

Product Details

Summary
Reactivity Hu, Mu(-), PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Endothelial Lipase Antibody Summary

Immunogen
A C-terminal synthetic peptide made to human Endothelial Lipase [UniProt# Q9Y5X9]
Localization
Secreted, cytoplasmic
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIPG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
This Endothelial Lipase antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. In Western blot a band is observed at ~57 kDa, representing the mature, non-glycosylated form of endothelial lipase protein. Another band is observed at ~30 kDa, representing the proteolytic form of the protein. In ICC/IF cytoplasmic staining was observed in HeLa cells.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24244566)
Control
Endothelial Lipase Overexpression Lysate
Control Peptide
Endothelial Lipase Peptide (NB400-111PEP)
Publications
Read Publications using
NB400-111 in the following applications:

Reactivity Notes

Human. Predicted to react with monkey based on 100% sequence homology. Does not appear to recognize mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Endothelial Lipase Antibody

  • EC 3.1.1
  • EC 3.1.1.3
  • EDLendothelial lipase
  • ELlipoprotein lipase H
  • Endothelial cell-derived lipase
  • lipase, endothelial
  • PRO719

Background

Endothelial lipase (EL) is an essential element of HDL metabolism which catalyzes the hydrolysis of HDL-phospholipids for facilitating their clearance from circulation. EL is synthesized in endothelial cells, however, is also expressed in other cell types such as coronary artery endothelial cells, umbilical vein endothelial cells, hepatocytes, osteosarcoma cell lines etc. with highest levels seen in organs including liver, placenta, lung, thyroid, kidney, testis and corpus luteum of ovary. EL is synthesized as a 55 kD protein, secreted as a 68 kD mature protein and after secretion, a portion of EL is cleaved and inactivated by convertases. Uncleaved EL is bound to heparan-sulfate proteoglycans on the cell surfaces where via its bridging functions, it facilitates HDL particle binding/uptake and selective uptake of HDL cholesteryl esters. Through its marked sn-1 phospholipase activity, EL cleaves lipoprotein-associated phospholipids, primarily HDL-phosphatidylethanolamine (HDL-PE) and -phosphatidylcholine (HDL-PC), generating mostly saturated free fatty acids and sn-1 lyso sn-2 acyl lysophospholipids (LysoPL). By its rather weak lysophospholipase activity, EL further hydrolyzes some of those EL-generated LPL, liberating unsaturated FFA. EL expression increases in cultured endothelial cells exposed to interleukin-1 beta or TNF-alpha as well as in vivo upon LPS exposure suggesting its role in inflammatory conditions. Accordingly, an elevated EL levels are found in patients with subclinical inflammation as encountered in the metabolic syndrome and coronary atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endothelial Lipase Antibody (NB400-111)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.


Filter By Application
ICC/IF
(1)
IHC-Fr
(1)
WB
(4)
All Applications
Filter By Species
Human
(5)
All Species

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Product General Protocols

View specific protocols for Endothelial Lipase Antibody (NB400-111): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endothelial Lipase Antibody (NB400-111) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-111AF350
Alexa Fluor 405 NB400-111AF405
Alexa Fluor 488 NB400-111AF488
Alexa Fluor 532 NB400-111AF532
Alexa Fluor 594 NB400-111AF594
Alexa Fluor 647 NB400-111AF647
Alexa Fluor 700 NB400-111AF700
Alexa Fluor 750 NB400-111AF750
Biotin NB400-111B
DyLight 350 NB400-111UV
DyLight 405 NB400-111V
DyLight 488 NB400-111G
DyLight 550 NB400-111R
DyLight 594 NB400-111DL594
DyLight 650 NB400-111C
DyLight 680 NB400-111FR
DyLight 755 NB400-111IR
FITC NB400-111F
HRP NB400-111H
Janelia Fluor 549 NB400-111JF549
Janelia Fluor 646 NB400-111JF646

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Bioinformatics

Gene Symbol LIPG