BAG2 Antibodies
We offer BAG2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each BAG2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our BAG2 Antibodies can be used in a variety of model species: Human, Rat. Use the list below to choose the BAG2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our BAG2 polyclonal antibodies and browse our BAG2 monoclonal antibody catalog.
Alternate Names for BAG2 Antibodies
anti-BAG2 antibody, anti-BAG family molecular chaperone regulator 2 antibody, anti-BAG-2 antibody, anti-BAG-family molecular chaperone regulator-2 antibody, anti-Bcl-2-associated athanogene 2 antibody, anti-BCL2-associated athanogene 2 antibody, anti-dJ417I1.2 (BAG-family molecular chaperone regulator 2) antibody, anti-dJ417I1.2 antibody, anti-KIAA0576 antibody, anti-MGC149462 antibody
10 Results for "bag2" in Primary Antibodies
![Western Blot: BAG2 Antibody [NB100-56087] - analysis of BAG-2 using this antibody. HeLa stably transfected Myc-tagged CHIP (<Carboxyl terminus of Hsp70-interacting protein) were immunoprecipitated with an anti-Myc antibody. Immunocomplexes were resolved by SDS-PAGE followed by analysis of BAG-2. This experiment was done to show the colocalization of CHIP and Bag-2. Please refer to Dai et al for additional information on HeLa cells stably transfected with Myc-CHIP.](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NB100-56087-img0003.jpg "Western Blot: BAG2 Antibody [NB100-56087] - analysis of BAG-2 using this antibody. HeLa stably transfected Myc-tagged CHIP (<Carboxyl terminus of Hsp70-interacting protein) were immunoprecipitated with an anti-Myc antibody. Immunocomplexes were resolved by SDS-PAGE followed by analysis of BAG-2. This experiment was done to show the colocalization of CHIP and Bag-2. Please refer to Dai et al for additional information on HeLa cells stably transfected with Myc-CHIP.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BAG2 Antibody [NB100-56087] - Immunofluorescence confocal microscopy of BAG-2 using NB100-56087 at 1:2000. HeLa cells stably transfected with Myc-CHIP (carboxyl terminus of Hsp70-interacting protein) and growing on coverslips were left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG-2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock. Please refer to Dai et al (2005) for additional information on HeLa cells stably transfected with Myc-CHIP.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56087-img0004.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody [NB100-56087] - BAG-2 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human BAG-2 polyclonal Antibody (Catalog # NB100-56087) at 1:1000 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NB100-56087-img0005.jpg)
![Western Blot: BAG2 Antibody [NBP1-59087] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP1-59087-img0005.jpg "Western Blot: BAG2 Antibody [NBP1-59087] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry-Paraffin: BAG2 Antibody [NBP1-59087] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-59087-img0003.jpg)
![Western Blot: BAG2 Antibody [NBP1-59087] - Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human.](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP1-59087-img0004.jpg)
![Western Blot: BAG2 Antibody [NBP1-59087] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP1-59087-img0001.jpg)
![Western Blot: BAG2 Antibody [NBP2-47544] - Analysis using Anti-BAG2 antibody NBP2-47544 (A) shows similar pattern to independent antibody NBP2-48541 (B).](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP2-47544-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BAG2 Antibody [NBP2-47544] - Analysis using Anti-BAG2 antibody NBP2-47544 (A) shows similar pattern to independent antibody NBP2-48541 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: BAG2 Antibody [NBP2-47544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47544-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BAG2 Antibody [NBP2-47544] - Staining in human smooth muscle and pancreas tissues using anti-BAG2 antibody. Corresponding BAG2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-47544-img0009.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody [NBP2-47544] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-47544-img0007.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody [NBP2-47544] - Staining of human smooth muscle shows high expression.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-47544-img0008.jpg)
![Western Blot: BAG2 Antibody (6D6Z0) [NBP3-16699] - Western blot analysis of extracts of various cell lines, using BAG2 antibody (NBP3-16699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/BAG2-Antibody-6D6Z0-Western-Blot-NBP3-16699-img0004.jpg "Western Blot: BAG2 Antibody (6D6Z0) [NBP3-16699] - Western blot analysis of extracts of various cell lines, using BAG2 antibody (NBP3-16699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: BAG2 Antibody (6D6Z0) [NBP3-16699] - Immunohistochemistry of paraffin-embedded mouse brain using BAG2 Rabbit mAb (NBP3-16699) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAG2-Antibody-6D6Z0-Immunohistochemistry-Paraffin-NBP3-16699-img0003.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody (6D6Z0) [NBP3-16699] - Immunohistochemistry of paraffin-embedded rat brain using BAG2 Rabbit mAb (NBP3-16699) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAG2-Antibody-6D6Z0-Immunohistochemistry-Paraffin-NBP3-16699-img0001.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody (6D6Z0) [NBP3-16699] - Immunohistochemistry of paraffin-embedded human esophageal cancer using BAG2 Rabbit mAb (NBP3-16699) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAG2-Antibody-6D6Z0-Immunohistochemistry-Paraffin-NBP3-16699-img0002.jpg)
![Western Blot: BAG2 Antibody (SR1371) [NBP3-22050] - Western blot analysis using NBP3-22050 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22050_rabbit-bag2-mab-sr1371-57202317215180.jpg "Western Blot: BAG2 Antibody (SR1371) [NBP3-22050] - Western blot analysis using NBP3-22050 in HepG2 cell lysate.")
![Western Blot: BAG2 Antibody [NB100-1776] - (0.3ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NB100-1776-img0002.jpg "Western Blot: BAG2 Antibody [NB100-1776] - (0.3ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: BAG2 Antibody [NB100-1776] - staining of Pig Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NB100-1776-img0004.jpg)
![Western Blot: BAG2 Antibody [NBP2-48541] - Analysis using Anti-BAG2 antibody NBP2-48541 (A) shows similar pattern to independent antibody NBP2-47544 (B).](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP2-48541-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BAG2 Antibody [NBP2-48541] - Analysis using Anti-BAG2 antibody NBP2-48541 (A) shows similar pattern to independent antibody NBP2-47544 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: BAG2 Antibody [NBP2-48541] - Staining of human smooth muscle shows distinct cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-NBP2-48541-img0001.jpg)
![Western Blot: BAG2 Antibody (29E9) [NBP2-59476] - Lane 1: Jurkat cell lysates, Lane 2: A549 cell lysates, Lane 3: K562 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: HeLa cell lysates](http://images.novusbio.com/fullsize/BAG2-Antibody-29E9-Western-Blot-NBP2-59476-img0001.jpg "Western Blot: BAG2 Antibody (29E9) [NBP2-59476] - Lane 1: Jurkat cell lysates, Lane 2: A549 cell lysates, Lane 3: K562 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: HeLa cell lysates")
![Immunocytochemistry/Immunofluorescence: BAG2 Antibody (29E9) [NBP2-59476] - Analysis of BAG2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human. BAG2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/BAG2-Antibody-29E9-Immunocytochemistry-Immunofluorescence-NBP2-59476-img0002.jpg)
![Western Blot: BAG2 Antibody (SR1374) [NBP3-21858] - Western blot analysis using NBP3-21858 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21858_rabbit-bag2-mab-sr1374-57202317215184.jpg "Western Blot: BAG2 Antibody (SR1374) [NBP3-21858] - Western blot analysis using NBP3-21858 in HeLa cell lysate.")
![Immunoprecipitation:BAG2 AntibodyNBP3-30610] -Detection of human BAG2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BAG2 antibody NBP3-30610 (lot NBP3-30610-1) used for IP at 6 µg per reaction. BAG2 was also immunoprecipitated by rabbit anti-BAG2. For blotting immunoprecipitated BAG2, NBP3-30610 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30610_rabbit-bag2-pab-3012202416181813.jpg "Immunoprecipitation:BAG2 AntibodyNBP3-30610] -Detection of human BAG2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BAG2 antibody NBP3-30610 (lot NBP3-30610-1) used for IP at 6 µg per reaction. BAG2 was also immunoprecipitated by rabbit anti-BAG2. For blotting immunoprecipitated BAG2, NBP3-30610 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")