PAMR1 Antibody

Western blot shows lysates of U2OS human osteosarcoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PAMR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6517) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PAMR1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PAMR1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PAMR1
  Leu22-Lys737
  Accession # NP_056245
• Specificity: Detects human PAMR1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PAMR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAMR1 Antibody

• DKFZP586H2123
• PAMR1
• peptidase domain containing associated with muscle regeneration 1
• Peptidase domain-containing protein associated with muscle regeneration 1
• RAMP
• RAMPinactive serine protease PAMR1
• regeneration associated muscle protease
• Regeneration-associated muscle protease homolog

Background

PAMR1 (Peptidase domain associated with muscle regeneration 1; also RAMP/ regeneration-associated muscle protease homolog) is a secreted, 80 kDa (predicted) member of the peptidase S1 family of enzymes. It is expressed by skeletal muscle, and appears to mediate skeletal muscle regeneration. Due to the lack of a catalytic serine at amino acid (aa) 672, PAMR1 demonstrates no enzyme activity. Mature PAMR1 is 716 aa in length (aa 22-737). It contains a Cys-rich segment (aa 42-94), one CUB domain (aa 128-234), an EGF-like region (aa 235-272), two SUSHI domains (aa 278-444) and a nonfunctional peptidase S1 domain (aa 445-737). There is one 17 aa deletion after Glu276. Over aa 21-737, human PAMR1 shares 88% aa identity with mouse PAMR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAMR1
• Uniprot:
  • NP_056245()