BAAT Antibody - BSA Free

Western Blot: BAAT Antibody [NBP2-15535] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: BAAT Antibody [NBP2-15535] - Human hepatoma, using BAAT antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BAAT Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BAAT Antibody - BSA Free (NBP2-15535) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human BAAT. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAAT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for BAAT Antibody - BSA Free

• BACAT
• BATbile acid-CoA:amino acid N-acyltransferase
• bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase)
• bile acid Coenzyme A: amino acid N-acyltransferase (glycineN-choloyltransferase)
• EC 2.3.1.65
• EC 3.1.2.2
• FLJ20300
• Glycine N-choloyltransferase
• Long-chain fatty-acyl-CoA hydrolase
• MGC104432

Background

The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: BAAT
• Uniprot:
  • Q14032(Human)