Immunocytochemistry/ Immunofluorescence: ATP7b Antibody [NB100-361] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
This ATP7b antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. By WB, this antibody recognizes a band at 165 kDa, representing ATP7b. This antibody also recognizes a band at ~220 kDa. This antibody may work on endogenous protein in immunohistochemistry, but it has only been tested on transfected ovarian carcinoma cells at a 1:500 dilution. However, NB100-360 may demonstrate less background for this assay.
165 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
ATP7b (copper-transporting P-type adenosine triphosphatase) is also called copper-transporting ATPase 2 or Wilson disease-associated protein and is involved in copper export out of the cells, such as the efflux of hepatic copper into the bile. ATP7b is a monomeric protein that interacts with COMMD1/MURR1, DCTN4 (in copper-dependent manner) and ATOX1. Predominantly localized in the trans-Golgi network (TGN), ATP7b is most abundantly expressed in liver, kidney and the brain tissues (isoform 2 is expressed in cytoplasm of brain cells but not in liver). Isoform 1 is proteolytically cleaved at N-terminus to produce the WND/140 kD form which is found in mitochondrions of hepatocytes as well as other tissue/cell types. Defects in ATP7B have been linked to Wilson disease (WD), an autosomal recessive disorder of copper metabolism, wherein, copper cannot be incorporated into hepatic ceruloplasmin and cannot be excreted from the liver into the bile.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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