ATP7b Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ATP7b Antibody [NB100-361] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu(-)Species Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

ATP7b Antibody Summary

Immunogen
A synthetic peptide made to an internal sequence near the C-terminus of human ATP7b. [UniProt# P35670]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP7B
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
This ATP7b antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. By WB, this antibody recognizes a band at 165 kDa, representing ATP7b. This antibody also recognizes a band at ~220 kDa. This antibody may work on endogenous protein in immunohistochemistry, but it has only been tested on transfected ovarian carcinoma cells at a 1:500 dilution. However, NB100-360 may demonstrate less background for this assay.
Theoretical MW
165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ATP7b Overexpression Lysate
Publications
Read Publications using
NB100-361 in the following applications:

  • WB
    3 publications

Reactivity Notes

Human and rat. Does not appear to react in mouse species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATP7b Antibody

  • ATPase, Cu(2+)- transporting, beta polypeptide
  • ATPase, Cu++ transporting, beta polypeptide
  • Copper pump 2
  • copper-transporting ATPase 2
  • EC 3.6.3
  • EC 3.6.3.4
  • PWD
  • WC1
  • WD
  • Wilson disease-associated protein
  • WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Background

ATP7b (copper-transporting P-type adenosine triphosphatase) is also called copper-transporting ATPase 2 or Wilson disease-associated protein and is involved in copper export out of the cells, such as the efflux of hepatic copper into the bile. ATP7b is a monomeric protein that interacts with COMMD1/MURR1, DCTN4 (in copper-dependent manner) and ATOX1. Predominantly localized in the trans-Golgi network (TGN), ATP7b is most abundantly expressed in liver, kidney and the brain tissues (isoform 2 is expressed in cytoplasm of brain cells but not in liver). Isoform 1 is proteolytically cleaved at N-terminus to produce the WND/140 kD form which is found in mitochondrions of hepatocytes as well as other tissue/cell types. Defects in ATP7B have been linked to Wilson disease (WD), an autosomal recessive disorder of copper metabolism, wherein, copper cannot be incorporated into hepatic ceruloplasmin and cannot be excreted from the liver into the bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATP7b Antibody (NB100-361)(3)

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Product General Protocols

View specific protocols for ATP7b Antibody (NB100-361): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATP7b Antibody (NB100-361) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-361AF350
Alexa Fluor 405 NB100-361AF405
Alexa Fluor 488 NB100-361AF488
Alexa Fluor 594 NB100-361AF594
Alexa Fluor 647 NB100-361AF647
Alexa Fluor 700 NB100-361AF700
Alexa Fluor 750 NB100-361AF750
Biotin NB100-361B
DyLight 350 NB100-361UV
DyLight 405 NB100-361V
DyLight 488 NB100-361G
DyLight 550 NB100-361R
DyLight 594 NB100-361DL594
DyLight 650 NB100-361C
DyLight 680 NB100-361FR
DyLight 755 NB100-361IR
FITC NB100-361F
HRP NB100-361H
Janelia Fluor 549 NB100-361JF549
Janelia Fluor 646 NB100-361JF646

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Contact Information

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Bioinformatics

Gene Symbol ATP7B