ATP7b Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Rt, Mu(-)Species Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

ATP7b Antibody [FITC] Summary

Immunogen
A synthetic peptide made to an internal sequence near the C-terminus of human ATP7b. [UniProt# P35670]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP7B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
Application Notes
This ATP7b antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. By WB, this antibody recognizes a band at 165 kDa, representing ATP7b. This antibody also recognizes a band at ~220 kDa. This antibody may work on endogenous protein in immunohistochemistry, but it has only been tested on transfected ovarian carcinoma cells at a 1:500 dilution. However, NB100-360 may demonstrate less background for this assay.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and rat. Does not appear to work in mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ATP7b Antibody [FITC]

  • ATPase, Cu(2+)- transporting, beta polypeptide
  • ATPase, Cu++ transporting, beta polypeptide
  • Copper pump 2
  • copper-transporting ATPase 2
  • EC 3.6.3
  • EC 3.6.3.4
  • PWD
  • WC1
  • WD
  • Wilson disease-associated protein
  • WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Background

ATP7b (copper-transporting P-type adenosine triphosphatase) is also called copper-transporting ATPase 2 or Wilson disease-associated protein and is involved in copper export out of the cells, such as the efflux of hepatic copper into the bile. ATP7b is a monomeric protein that interacts with COMMD1/MURR1, DCTN4 (in copper-dependent manner) and ATOX1. Predominantly localized in the trans-Golgi network (TGN), ATP7b is most abundantly expressed in liver, kidney and the brain tissues (isoform 2 is expressed in cytoplasm of brain cells but not in liver). Isoform 1 is proteolytically cleaved at N-terminus to produce the WND/140 kD form which is found in mitochondrions of hepatocytes as well as other tissue/cell types. Defects in ATP7B have been linked to Wilson disease (WD), an autosomal recessive disorder of copper metabolism, wherein, copper cannot be incorporated into hepatic ceruloplasmin and cannot be excreted from the liver into the bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP7B