ATM [p Ser1981] Antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda ‑phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ATM (S1981) at approximately 270 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ATM [p Ser1981] Antibody Summary

• Immunogen: Phosphopeptide containing human ATM S1981 site
• Modification: p Ser1981
• Specificity: Detects human ATM when phosphorylated at S1981. Also detects the comparable phosphorylated sites in mouse ATM (S1987) and rat ATM (S1952).
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ATM [p Ser1981] Antibody

• AT mutated
• A-T mutated
• AT1
• ATA
• ataxia telangiectasia mutated (includes complementation groups A, C and D)
• ataxia telangiectasia mutated
• ATC
• ATD
• ATDC
• ATE
• ATM serine/threonine kinase
• ATM
• DKFZp781A0353
• EC 2.7.11.1
• MGC74674
• serine-protein kinase ATM
• TEL1
• TEL1, telomere maintenance 1, homolog
• TELO1
• TPLL

Background

The Ataxia telangiectasia-mutated (ATM) protein kinase exists as a dimer in the cell nucleus. Changes in DNA structure induced by genotoxic stress lead to activation of ATM and phosphorylation of S1981 in trans. Once S1981 is phosphorylated, the dimer dissociates and active ATM monomers signal to downstream targets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATM Antibody (AF1655)(12)

Publications using AF1655

• SH Park, N Kang, E Song, M Wie, EA Lee, S Hwang, D Lee, JS Ra, IB Park, J Park, S Kang, JH Park, S Hohng, KY Lee, K Myung ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress Nat Commun, 2019;10(1):5718. 2019 [PMID: 31844045] (Western Blot, Human)
• Ji-Hoon Lee, Michael R Mand, Chung-Hsuan Kao, Yi Zhou, Seung W Ryu, Alicia L Richards, Joshua J Coon, Tanya T Paull ATM directs DNA damage responses and proteostasis via genetically separable pathways. Science Signaling, 2018;0(0):1937-9145. 2018 [PMID: 29317520] (Bioassay, Human)
• EA Smith, B Gole, NA Willis, R Soria, LM Starnes, EF Krumpelbec, AG Jegga, AM Ali, H Guo, AR Meetei, PR Andreassen, F Kappes, LM Vinnedge, JA Daniel, R Scully, L Wiesmüller, SI Wells DEK is required for homologous recombination repair of DNA breaks Sci Rep, 2017;7(0):44662. 2017 [PMID: 28317934] (Western Blot, Human)
• TL Clarke, MP Sanchez-Ba, K Chiang, JJ Reynolds, J Herrero-Ru, TM Bandeiras, PM Matias, SL Maslen, JM Skehel, GS Stewart, CC Davies PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination Mol. Cell, 2017;0(0):. 2017 [PMID: 28238654] (Western Blot, Human)
• Cornell L, Munck J, Alsinet C, Villanueva A, Ogle L, Willoughby C, Televantou D, Thomas H, Jackson J, Burt A, Newell D, Rose J, Manas D, Shapiro G, Curtin N, Reeves H DNA-PK-A candidate driver of hepatocarcinogenesis and tissue biomarker that predicts response to treatment and survival. Clin Cancer Res, 2015;21(4):925-33. 2015 [PMID: 25480831] (IHC-P, Human)
• Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender S, Fullard N, Nelson G, Mann J, van de Sluis B, Mann D, von Zglinicki T Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nat Commun, 2014;2(0):4172. 2014 [PMID: 24960204] (Western Blot, Mouse)
• Lee, Ji-Hoon, Guo, Zhi, Myler, Logan R, Zheng, Suting, Paull, Tanya T Direct activation of ATM by resveratrol under oxidizing conditions. PLoS ONE, 2014;9(6):e97969. 2014 [PMID: 24933654] (Western Blot, Human)
• Byrd PJ, Srinivasan V, Last JI Severe reaction to radiotherapy for breast cancer as the presenting feature of ataxia telangiectasia. Br. J. Cancer, 2012;106(2):262-8. 2012 [PMID: 22146522] (Western Blot, Human)
• Reiman A, Srinivasan V, Barone G Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours. Br. J. Cancer, 2011;105(4):586-91. 2011 [PMID: 21792198] (Western Blot, Human)
• Rajesh P, Rajesh C, Wyatt MD RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine. DNA Repair (Amst.), 2010;9(4):458-67. 2010 [PMID: 20133210] (Western Blot, Mouse)
• Jia XZ, Yang SY, Zhou J, Li SY, Ni JH, An GS, Jia HT Inhibition of CHK1 kinase by Go6976 converts 8-chloro-adenosine-induced G2/M arrest into S arrest in human myelocytic leukemia K562 cells. Biochem. Pharmacol., 2009;77(5):770-80. 2009 [PMID: 19059218] (Western Blot, Human)
• Leonard JM, Ye H, Wetmore C, Karnitz LM Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability. J. Cell Biol., 2008;183(3):385-91. 2008 [PMID: 18955550] (Western Blot, Mouse)

FAQs for ATM Antibody (AF1655). (Showing 1 - 1 of 1 FAQs).

1. What is the theoretical molecular weight for your ATM antibodies?
   • The theoretical molecular weight for our ATM antibodies is 351 kDa.

Bioinformatics

• Gene Symbol: ATM