ARNT/HIF-1 beta Antibody (H1beta234) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ft, Pm, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
H1beta234
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

ARNT/HIF-1 beta Antibody (H1beta234) [DyLight 405] Summary

Immunogen
Fusion protein containing amino acids 496-789 of human HIF-1 beta. [UniProt# P27540]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ARNT
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This HIF-1 beta (H1beta234) antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections and Chromatin Immunoprecipitation. By Western blot a band at approximately 92 kDa is seen. Use in Gel Shift Super Assay reported in scientific literature (PMID: 11325839).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ARNT/HIF-1 beta Antibody (H1beta234) [DyLight 405]

  • ARNT protein
  • ARNT
  • aryl hydrocarbon receptor nuclear translocator
  • BHLHE2
  • HIF1 beta
  • HIF-1 beta
  • HIF1B
  • HIF1BETA
  • HIF-1-beta
  • HIF1-beta
  • hypoxia-inducible factor 1, beta subunit
  • Hypoxia-inducible factor 1-beta
  • nuclear translocator
  • TANGO

Background

HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits which are bHLH proteins of PAS (PER, ARNT, SIM) family. HIF-1 beta forms heterodimer with AHR, AHRR, HIF1 alpha and EPAS1/HIF2 alpha as well as with other bHLH proteins and its dimerization is required for efficient DNA binding. It has been shown to interact with TACC3 and NOCA7 also. HIF-1 beta is also known as ARNT (AhR nuclear translocator) due to its ability to partner with AhR to form a heterodimeric transcription factor complex which is required for AhR activity. It is essential for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding and the AhR-HIF-1 beta complex then initiates transcription of genes involved in xenobiotic transformation, especially of PAHs. HIF-1 beta heterodimer with HIF1 alpha or EPAS1/HIF2 alpha functions as a transcriptional regulator of the adaptive response to hypoxia. Moreover, a chromosomal translocation resulting in TEL-ARNT fusion protein is associated with AML (acute myeloblastic leukemia) and decreased HIF-1 beta expression levels in pancreatic islets from NIDDM subjects reflects its important role in beta-cell function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-124B
DyLight 488 Labeled NB100-124G
DyLight 550 Labeled NB100-124R
DyLight 650 Labeled NB100-124C
FITC Labeled NB100-124F
HRP Labeled NB100-124H

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Bioinformatics Tool for ARNT/HIF-1 beta Antibody (NB100-124V)

Discover related pathways, diseases and genes to ARNT/HIF-1 beta Antibody (NB100-124V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on ARNT/HIF-1 beta.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

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Bioinformatics

Gene Symbol ARNT
Entrez