SIM2 Antibody

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Western Blot: SIM2 Antibody [NBP1-68864] - (0.5ug/ml) staining of fetal Mouse kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SIM2 Antibody Summary

Immunogen
Peptide with sequence C-SDLLYTPSYS corresponding to internal region according to NP_033664.2.
Epitope
C-SDLLYTPSYS
Specificity
This antibody is expected to recognize all reported isoforms (NP_005060.1; NP_033664.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SIM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: Approx. 80 kDa band observed in fetal mouse kidney lysates (calculated MW of 73.2 kDa band according to human NP_005060.1) and 72.5 kDa band according to mouse NP_035507.2.
Publications
Read Publication using NBP1-68864.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIM2 Antibody

  • human transcription factor SIM2, homolog of the Drosophila single-minded geneSIM110BHLHE15
  • short isoform
  • single-minded (Drosophila) homolog 2
  • single-minded homolog 2 (Drosophila)
  • transcription factor SIM2

Background

SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. The Drosophila sim gene encodes a transcription factor that is a master regulator of fruit fly neurogenesis. SIM2 maps within the so-called Down syndrome chromosomal region. Based on the mapping position, its potential function as transcriptional repressor and similarity to Drosophila sim, it is proposed that SIM2 may contribute to some specific Down syndrome phenotypes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIM2 Antibody (NBP1-68864)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SIM2
Entrez
Uniprot