SIM2 Antibody

Western Blot: SIM2 Antibody [NBP1-68864] - (0.5ug/ml) staining of fetal Mouse kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu, Rt
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SIM2 Antibody Summary

• Immunogen: Peptide with sequence C-SDLLYTPSYS corresponding to internal region according to NP_033664.2.
• Epitope: C-SDLLYTPSYS
• Specificity: This antibody is expected to recognize all reported isoforms (NP_005060.1; NP_033664.2).
• Predicted Species: Human (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 80 kDa band observed in fetal mouse kidney lysates (calculated MW of 73.2 kDa band according to human NP_005060.1) and 72.5 kDa band according to mouse NP_035507.2.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SIM2 Antibody

• human transcription factor SIM2, homolog of the Drosophila single-minded geneSIM110BHLHE15
• short isoform
• single-minded (Drosophila) homolog 2
• single-minded homolog 2 (Drosophila)
• transcription factor SIM2

Background

SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. The Drosophila sim gene encodes a transcription factor that is a master regulator of fruit fly neurogenesis. SIM2 maps within the so-called Down syndrome chromosomal region. Based on the mapping position, its potential function as transcriptional repressor and similarity to Drosophila sim, it is proposed that SIM2 may contribute to some specific Down syndrome phenotypes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIM2 Antibody (NBP1-68864)(1)

Publication using NBP1-68864

• Fei Q, Wu ZH, Zhou X et al. [Association study of SIM2 gene polymorphisms with susceptibility to congenital scoliosis in a Chinese Han population] Zhonghua Yi Xue Za Zhi 2009-11-10 [PMID: 20137643]

Bioinformatics

• Gene Symbol: SIM2
• Entrez:
  • 6493(Human)
  • 20465(Mouse)
  • 304071(Rat)
• Uniprot:
  • NP_005060.1(Human)