PBR Antibody

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Western Blot: PBR Antibody [NB100-41398] - Western blot analysis of PBR in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NB100-41398 at 0.1ug/ml. Primary incubation was 1 hour. Detected by ...read more
Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - IHC staining (2.5 ug/ml) of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PBR Antibody Summary

Immunogen
Peptide with sequence C-RDNHGWRGGRRLPE corresponding to C-Terminus according to NP_000705.2.
Specificity
This antibody is expected to recognise one isoform (NP_000705.2) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TSPO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 17 kDa band observed in human breast cancer lysates (calculated MW of 18.8 kDa band according to NP_000705.2). IHC-P: Human colon shows strong cytoplasm staining of absorptive cells. ICC/IF usage is reproted in scientific literature (PMID: 23967175).
Theoretical MW
18.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PBR Lysate (NBL1-17384)
PBR Lysate (NBP2-64860)
Publications
Read Publications using
NB100-41398 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PBR Antibody

  • benzodiazepine peripheral binding site
  • BZRPDBI
  • IBP
  • MBRPBS
  • mDRC
  • Mitochondrial benzodiazepine receptor
  • PBRBPBS
  • PBRS
  • peripheral benzodiazepine receptor
  • Peripheral-type benzodiazepine receptor
  • pk18
  • PKBSbenzodiazapine receptor (peripheral)
  • PTBR
  • translocator protein (18kDa)
  • translocator protein

Background

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PBR Antibody (NB100-41398)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-41398 Applications Species
Buck JR, McKinley ET, Fu A et al. Preclinical TSPO Ligand PET to Visualize Human Glioma Xenotransplants: A Preliminary Study. PLoS ONE. 2015 Oct 31 [PMID: 26517124] (IHC-P, Human) IHC-P Human
Zhou T, Frabutt DA, Moremen KW, Zheng YH et al. ERManI is required for HIV-1 envelope glycoprotein degradation via endoplasmic reticulum-associated protein degradation pathway J. Biol. Chem. 2015 Jul 23 [PMID: 26205822] (WB, Human) WB Human
Zhou T, Dang Y, Zheng YH. The Mitochondrial Translocator Protein, TSPO, Inhibits HIV-1 Envelope Glycoprotein Biosynthesis via the Endoplasmic Reticulum-Associated Protein Degradation Pathway. J. Virol. 2014 Mar 1 [PMID: 24403586] (WB, Human) WB Human
Wu X, Gallo KA. The 18-kDa Translocator Protein (TSPO) Disrupts Mammary Epithelial Morphogenesis and Promotes Breast Cancer Cell Migration. PLoS One. 2013 Aug 14 [PMID: 23967175] (WB, ICC/IF, Human) WB, ICC/IF Human
Joo, HK et al. Peripheral benzodiazepine receptor regulates vascular endothelial activations via suppression of the voltage-dependent anion channel-1 FEBS Lett 2012 [PMID: 22616995] (WB, Human) WB Human
Fafalios A, Akhavan A, Parwani AV et al. Translocator Protein Blockade Reduces Prostate Tumor Growth. Clin Cancer Res;15(19):6177-6184. 2009 [PMID: 19789311]
Papadopoulos V, Baraldi M, Guilarte TR et al. Translocator protein (18kDa): new nomenclature for the peripheral-typebenzodiazepine receptor based on its structure and molecular function. Trends Pharmacol Sci 2006 Aug [PMID: 16822554]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TSPO
Entrez
Uniprot