PBR Antibody

Images

 
Western Blot: PBR Antibody [NB100-41398] - Staining of A431 cell lysate.(35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PBR Antibody [NB100-41398] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: PBR Antibody [NB100-41398] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Immunocytochemistry/ Immunofluorescence: PBR Antibody [NB100-41398] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PBR Antibody Summary

Immunogen
Peptide with sequence C-RDNHGWRGGRRLPE corresponding to C-Terminus according to NP_000705.2.
Specificity
This antibody is expected to recognise one isoform (NP_000705.2) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TSPO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/mL
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
Theoretical MW
18.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PBR Overexpression Lysate
PBR Knockout HeLa Cell Lysate
Publications
Read Publications using
NB100-41398 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31353076).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PBR Antibody

  • benzodiazepine peripheral binding site
  • BZRPDBI
  • IBP
  • MBRPBS
  • mDRC
  • Mitochondrial benzodiazepine receptor
  • PBR
  • PBRBPBS
  • PBRS
  • peripheral benzodiazepine receptor
  • Peripheral-type benzodiazepine receptor
  • pk18
  • PKBSbenzodiazapine receptor (peripheral)
  • PTBR
  • translocator protein (18kDa)
  • translocator protein
  • TSPO

Background

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PBR Antibody (NB100-41398)(9)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
IP
(1)
WB
(4)
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Human
(6)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-41398 Applications Species
Hieu Tran V, Park H, Park J et al. Synthesis and evaluation of novel potent TSPO PET ligands with 2-phenylpyrazolo[1,5-a]pyrimidin-3-yl acetamide Bioorg. Med. Chem. Jul 19 2019 [PMID: 31353076] (IHC, Rat, Human) IHC Rat, Human
Robb J Characterisation of immunometabolic responses in astrocytes Thesis Jan 1 2020 (IP) IP
Buck JR, McKinley ET, Fu A et al. Preclinical TSPO Ligand PET to Visualize Human Glioma Xenotransplants: A Preliminary Study. PLoS ONE. 2015 Oct 31 [PMID: 26517124] (IHC-P, Human) IHC-P Human
Zhou T, Frabutt DA, Moremen KW, Zheng YH et al. ERManI is required for HIV-1 envelope glycoprotein degradation via endoplasmic reticulum-associated protein degradation pathway J. Biol. Chem. 2015 Jul 23 [PMID: 26205822] (WB, Human) WB Human
Zhou T, Dang Y, Zheng YH. The Mitochondrial Translocator Protein, TSPO, Inhibits HIV-1 Envelope Glycoprotein Biosynthesis via the Endoplasmic Reticulum-Associated Protein Degradation Pathway. J. Virol. 2014 Mar 1 [PMID: 24403586] (WB, Human) WB Human
Wu X, Gallo KA. The 18-kDa Translocator Protein (TSPO) Disrupts Mammary Epithelial Morphogenesis and Promotes Breast Cancer Cell Migration. PLoS One. 2013 Aug 14 [PMID: 23967175] (WB, ICC/IF, Human) WB, ICC/IF Human
Joo, HK et al. Peripheral benzodiazepine receptor regulates vascular endothelial activations via suppression of the voltage-dependent anion channel-1 FEBS Lett 2012 [PMID: 22616995] (WB, Human) WB Human
Fafalios A, Akhavan A, Parwani AV et al. Translocator Protein Blockade Reduces Prostate Tumor Growth. Clin Cancer Res;15(19):6177-6184. 2009 [PMID: 19789311]
Papadopoulos V, Baraldi M, Guilarte TR et al. Translocator protein (18kDa): new nomenclature for the peripheral-typebenzodiazepine receptor based on its structure and molecular function. Trends Pharmacol Sci 2006 Aug [PMID: 16822554]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol TSPO
Entrez
Uniprot