PBR Antibody

Western Blot: PBR Antibody [NB100-41398] - Staining of A431 cell lysate.(35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: PBR Antibody [NB100-41398] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry: PBR Antibody [NB100-41398] - Correlative TSPO immunohistochemistry in a primary human grade III astrocytoma xenotransplant, Tumor + White Matter Tract (40X). Image collected and cropped by CiteAb from the following publication (www.journals.plos.org/plosone/article?id=10.1371/journal.pone.0141659) licensed under a CC-BY license.

Flow Cytometry: PBR Antibody [NB100-41398] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: PBR Antibody [NB100-41398] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative cLontrol: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry: PBR Antibody [NB100-41398] - Human tumor microarrays (TMAs). Pilocytic astrocytoma TSPO immunohistochemistry (A) and Glioblastoma multiforme TSPO immunohistochemistry (B). Image collected and cropped by CiteAb from the following publication (www.journals.plos.org/plosone/article?id=10.1371/journal.pone.0141659) licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PBR Antibody Summary

• Immunogen: Peptide with sequence C-RDNHGWRGGRRLPE corresponding to C-Terminus according to NP_000705.2.
• Specificity: This antibody is expected to recognise one isoform (NP_000705.2) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TSPO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 2-4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA detection limit 1:128000
  • Western Blot 0.1-0.3 ug/ml
• Theoretical MW: 18.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PBR Overexpression Lysate

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33725191).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PBR Antibody

• benzodiazepine peripheral binding site
• BZRPDBI
• IBP
• MBRPBS
• mDRC
• Mitochondrial benzodiazepine receptor
• PBR
• PBRBPBS
• PBRS
• peripheral benzodiazepine receptor
• Peripheral-type benzodiazepine receptor
• pk18
• PKBSbenzodiazapine receptor (peripheral)
• PTBR
• translocator protein (18kDa)
• translocator protein
• TSPO

Background

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PBR Antibody (NB100-41398)(13)

Publications using NB100-41398

• Nutma E, Gebro E, Marzin MC et al. Activated microglia do not increase 18 kDa translocator protein (TSPO) expression in the multiple sclerosis brain Glia 2021-06-19 [PMID: 34145928]
• Cohen AS, Li J, Hight MR et al. TSPO-targeted PET and Optical Probes for the Detection and Localization of Premalignant and Malignant Pancreatic Lesions Clinical Cancer Research 2020-11-15 [PMID: 32933996] (IHC)
• Hieu Tran V, Park H, Park J et al. Synthesis and evaluation of novel potent TSPO PET ligands with 2-phenylpyrazolo[1,5-a]pyrimidin-3-yl acetamide Bioorg. Med. Chem. 2019-07-19 [PMID: 31353076] (IF/IHC, Rat, Human)
• Buck JR, McKinley ET, Fu A et al. Preclinical TSPO Ligand PET to Visualize Human Glioma Xenotransplants: A Preliminary Study. PLoS ONE. 2015-10-31 [PMID: 26517124] (IHC-P, Human)
• Zhou T, Frabutt DA, Moremen KW, Zheng YH et al. ERManI is required for HIV-1 envelope glycoprotein degradation via endoplasmic reticulum-associated protein degradation pathway J. Biol. Chem. 2015-07-23 [PMID: 26205822] (WB, Human)
• Zhou T, Dang Y, Zheng YH. The Mitochondrial Translocator Protein, TSPO, Inhibits HIV-1 Envelope Glycoprotein Biosynthesis via the Endoplasmic Reticulum-Associated Protein Degradation Pathway. J. Virol. 2014-03-01 [PMID: 24403586] (WB, Human)
• Wu X, Gallo KA. The 18-kDa Translocator Protein (TSPO) Disrupts Mammary Epithelial Morphogenesis and Promotes Breast Cancer Cell Migration. PLoS One. 2013-08-14 [PMID: 23967175] (WB, ICC/IF, Human)
• Joo, HK et al. Peripheral benzodiazepine receptor regulates vascular endothelial activations via suppression of the voltage-dependent anion channel-1 FEBS Lett 2012-01-01 [PMID: 22616995] (WB, Human)
• Papadopoulos V, Baraldi M, Guilarte TR et al. Translocator protein (18kDa): new nomenclature for the peripheral-typebenzodiazepine receptor based on its structure and molecular function. Trends Pharmacol Sci 2006-08-01 [PMID: 16822554]
• Robb J Characterisation of immunometabolic responses in astrocytes Thesis 2020-01-01 (IP)
• Chang CW, Chiu CH, Lin MH et al. GMP-compliant fully automated radiosynthesis of [18F]FEPPA for PET/MRI imaging of regional brain TSPO expression EJNMMI research 2021-03-16 [PMID: 33725191] (WB, ICC/IF, Mouse)
• Nutma E, Fancy N, Marzin M et al. Translocator protein is a marker of activated microglia in rodent models but not human neurodegenerative diseases Research Square 2022-03-23
• Fafalios A, Akhavan A, Parwani AV et al. Translocator Protein Blockade Reduces Prostate Tumor Growth. Clin Cancer Res;15(19):6177-6184. 2009-01-01 [PMID: 19789311]

Bioinformatics

• Gene Symbol: TSPO
• Entrez:
  • 706(Human)
• Uniprot:
  • P30536()