HSP90 alpha Antibody

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Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.
Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein 86 (HSP86, green) in HeLa cells and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton ...read more
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.
Western Blot: HSP90 alpha Antibody [NB120-2928] - Figure 1 shows a Western blot of mouse HSP84 and HSP86 using NB120-2928 and NB120-2927 respectively.
Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of epidermoid carcinoma (A431) cells using Heat Shock Protein 86 Polyclonal Antibody. Decreasing amounts of A431 whole cell lysates were probed with PA3-013 at ...read more
Western Blot: HSP90 alpha Antibody [NB120-2928] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of ...read more
Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Analysis of differentiated colon adenocarcinoma from human colon tissue sections. To expose target proteins, heat-induced epitope retrieval was ...read more
Immunoprecipitation: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein (HSP86) was performed on HeLa cells. Antigen-antibody complexes formed by incubating 500ug whole cell lysate with 2ug of HSP86 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Rb, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HSP90 alpha Antibody Summary

Immunogen
Synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86.
Localization
Cell Membrane and Nuclear
Specificity
Detects Heat Shock Protein 86 (HSP 86). This does not detect HSP 84.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSP90AA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 2 ug
Application Notes
IP: HSP 86 exists primarily as homodimers in HeLa cells. This antibody is capable of precipitating HSP 86 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.
Publications
Read Publications using
NB120-2928 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 21129982).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP90 alpha Antibody

  • FLJ31884
  • Heat shock 86 kDa
  • heat shock 90kD protein 1, alpha
  • heat shock 90kD protein 1, alpha-like 4
  • heat shock 90kD protein, alpha-like 4
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
  • heat shock protein HSP 90-alpha
  • HSP86
  • Hsp89
  • HSP89A
  • HSP90 alpha
  • Hsp90
  • HSP90A
  • HSP90AA1
  • HSP90AHSP86
  • HSP90N
  • HSPC1
  • HSPC1HSP 86
  • HSPCA
  • HSPCAHSP89A
  • HSPCAL1
  • HSPCAL4
  • HSPN
  • LAP2
  • Renal carcinoma antigen NY-REN-38

Background

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP 90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP 90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, Ah receptor, and some kinases. Studies have shown that murine HSP 90 exists as two forms, HSP 84 and HSP 86, coded by related but separate genes, with 86% homologous amino acid sequences. These forms are analogous to the two forms of human HSP 90, HSP 89 beta and HSP 89 alpha. In an unstressed mouse fibroblast, the basal level of HSP 84 is found to be double that of HSP 86. However, after heat shock, HSP 86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP 86, but not HSP 84, decreases. HSP 84 and HSP 86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP90 alpha Antibody (NB120-2928)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, IHC-P.


Filter By Application
IHC
(1)
IHC-P
(2)
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Filter By Species
Mouse
(3)
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Showing Publications 1 - 7 of 7.
Publications using NB120-2928 Applications Species
Deneka AY, Haber L, Kopp MC et al. Tumor-targeted SN38 inhibits growth of early stage non-small cell lung cancer (NSCLC) in a KRas/p53 transgenic mouse model. PLoS ONE Apr 28 2017 [PMID: 28453558] (IHC-P, Mouse) IHC-P Mouse
Smithline Zachary B, Nikonova Anna S, Hensley Harvey H et al. Inhibiting heat shock protein 90 (HSP90) limits the formation of liver cysts induced by conditional deletion of Pkd1 in mice. PLoS One. 2014 Dec 04 [PMID: 25474361] (IHC, Mouse) IHC Mouse
Seeger-Nukpezah T, Proia DA, Egleston BL et al. Inhibiting the HSP90 chaperone slows cyst growth in a mouse model of autosomal dominant polycystic kidney disease. Proc Natl Acad Sci U S A 2013 Jul 30 [PMID: 23858461] (IHC-P, Mouse) IHC-P Mouse
Shelton SN, Shawgo ME, Matthews SB et al. KU135, a novel novobiocin-derived C-terminal inhibitor of the 90-kDa heat shock protein, exerts potent antiproliferative effects in human leukemic cells. Mol Pharmacol. 2009 Dec. [PMID: 19741006]
De Nardo D, Masendycz P, Ho S et al. A central role for the Hsp90.Cdc37 molecular chaperone module in interleukin-1 receptor-associated-kinase-dependent signaling by toll-like receptors. J Biol Chem. 2005 Mar. [PMID: 15647277]
Peterson JK, Moran F, Conley AJ et al. Zonal expression of endothelial nitric oxide synthase in sheep and rhesus adrenal cortex. Endocrinology. 2001 Dec. [PMID: 11713235]
Perdew GH, Hord N, Hollenback CE et al. Localization and characterization of the 86- and 84-kDa heat shock proteins in Hepa 1c1c7 cells. Exp Cell Res. 1993 Dec. [PMID: 8262153]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Blogs on HSP90 alpha.

Hsp90A - helping to fold a wide variety of signal transduction proteins
Heat-shock protein 90 (Hsp90) is a conserved ATP-dependent chaperone with diverse cellular functions. Hsp90 is ubiquitously expressed but is further induced upon cellular stress such as heat-shock or nutrient deprivation. Hsp90 is essential for the...  Read full blog post.

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Bioinformatics

Gene Symbol HSP90AA1
Entrez
Uniprot