Arginase 1/ARG1/liver Arginase Antibody [Unconjugated]

Western blot shows lysates of human liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western lane view shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Arginase 1/ARG1/liver Arginase Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Arginase 1/ARG1
  Met1-Lys322
  Accession # P05089
• Specificity: Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ARG1

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunoprecipitation 25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Arginase 1/ARG1/liver Arginase Antibody [Unconjugated]

• AI
• ARG1
• Arginase 1
• arginase, liver
• Arginase-1
• EC 3.5.3.1
• Liver Arginase
• Liver-type arginase
• PGIF
• Type I Arginase

Background

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arginase 1/ARG1/liver Arginase Antibody (AF5868)(8)

Publications using AF5868

• W Ren, J Hou, C Yang, H Wang, S Wu, Y Wu, X Zhao, C Lu Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res., 2019;38(1):62. 2019 [PMID: 30736829] (Western Blot, Xenograft)
• H Gerber, S Mosser, B Boury-Jamo, M Stumpe, A Piersigill, C Goepfert, J Dengjel, U Albrecht, F Magara, PC Fraering The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease Acta Neuropathol Commun, 2019;7(1):13. 2019 [PMID: 30704515] (Western Blot, Mouse)
• J Wosik, W Chen, K Qin, RM Ghobrial, JZ Kubiak, M Kloc Magnetic Field Changes Macrophage Phenotype Biophys. J., 2018;114(8):2001-2013. 2018 [PMID: 29694876] (Western Blot, Mouse)
• J Suwanpradi, M Shih, L Pontius, B Yang, A Birukova, E Guttman-Ya, DL Corcoran, LG Que, RM Tighe, AS MacLeod Arginase1 Deficiency in Monocytes/Macrophages Upregulates Inducible Nitric Oxide Synthase To Promote Cutaneous Contact Hypersensitivity J. Immunol., 2017;0(0):. 2017 [PMID: 28747341] (Flow Cytometry, Mouse)
• P Maliver, M Festag, M Bennecke, F Christen, B Bánfai, B Lenz, M Winter Assessment of Preclinical Liver and Skeletal Muscle Biomarkers Following Clofibrate Administration in Wistar Rats Toxicol Pathol, 2017;0(0):1926233177072. 2017 [PMID: 28485676] (ELISA Development (Capture), Rat)
• DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population Nat Commun, 2017;8(0):14919. 2017 [PMID: 28374774] (Western Blot, Mouse)
• J Wagener, DM MacCallum, GD Brown, NA Gow Candida albicans Chitin Increases Arginase-1 Activity in Human Macrophages, with an Impact on Macrophage Antimicrobial Functions MBio, 2017;8(1):. 2017 [PMID: 28119468] (Western Blot, Human)
• Liesz A, Dalpke A, Mracsko E, Antoine D, Roth S, Zhou W, Yang H, Na S, Akhisaroglu M, Fleming T, Eigenbrod T, Nawroth P, Tracey K, Veltkamp R DAMP signaling is a key pathway inducing immune modulation after brain injury. J Neurosci, 2015;35(2):583-98. 2015 [PMID: 25589753] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ARG1
• Uniprot:
  • P05089()