Arginase 1/ARG1/liver Arginase Antibody [Phycoerythrin]

HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Phycoerythrin

Arginase 1/ARG1/liver Arginase Antibody [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human Arginase 1/ARG1
  Met1-Lys322
  Accession # P05089
• Specificity: Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ARG1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Arginase 1/ARG1/liver Arginase Antibody [Phycoerythrin]

• AI
• ARG1
• Arginase 1
• arginase, liver
• Arginase-1
• EC 3.5.3.1
• EC:3.5.3.1
• Liver Arginase
• Liver-type arginase
• PGIF
• Type I Arginase

Background

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arginase 1/ARG1/liver Arginase Antibody (IC5868P)(43)

Publications using IC5868P

• Perez-Penco, M;Byrdal, M;Lara de la Torre, L;Ballester, M;Khan, S;Siersbæk, M;Lecoq, I;Madsen, CO;Kjeldsen, JW;Svane, IM;Hansen, M;Donia, M;Johansen, JS;Olsen, LR;Grøntved, L;Chen, IM;Arnes, L;Holmström, MO;Andersen, MH; The antitumor activity of TGF?-specific T cells is dependent on IL-6 signaling Cellular and Molecular Immunology 2024-12-09 [PMID: 39653766] (Flow Cytometry, Mouse)
• Wang, W;Ren, X;Chen, X;Hong, Q;Cai, G; Integrin ?1-rich extracellular vesicles of kidney recruit Fn1+ macrophages to aggravate ischemia-reperfusion-induced inflammation JCI insight 2024-01-23 [PMID: 38258908] (Flow Cytometry, Mouse)
• Liu, Y;Luo, X;Chen, Y;Dang, J;Zeng, D;Guo, X;Weng, W;Zhao, J;Shi, X;Chen, J;Dong, B;Zhong, S;Ren, J;Li, Y;Wang, J;Zhang, J;Sun, J;Xu, H;Lu, Y;Brand, D;Zheng, SG;Pan, Y; Heterogeneous ferroptosis susceptibility of macrophages caused by focal iron overload exacerbates rheumatoid arthritis Redox biology 2023-12-20 [PMID: 38142586] (Flow Cytometry, Mouse)
• Jiao, S;Li, C;Guo, F;Zhang, J;Zhang, H;Cao, Z;Wang, W;Bu, W;Lin, M;Lü, J;Zhou, Z; SUN1/2 controls macrophage polarization via modulating nuclear size and stiffness Nature communications 2023-10-12 [PMID: 37828059] (Flow Cytometry, Mouse)
• Zhou J, Wang H, Shu T et al. Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis iScience 10/20/2023 [PMID: 37731616]
• Zhou J, Wang H, Shu T et al. Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis iScience 2023-10-20 [PMID: 37731616]
• Dongsheng Guan, Yingxia Li, Yinglin Cui, Huanghong Zhao, Ning Dong, Kun Wang et al. 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction Acta Biochimica et Biophysica Sinica 8/1/2023 [PMID: 37431183]
• Gu, C;Chen, M;Li, X;Geng, D;Wang, C; MAGL regulates synovial macrophage polarization vis inhibition of mitophagy in osteoarthritic pain Molecular medicine reports 2023-06-01 [PMID: 37144506] (Flow Cytometry, Mouse)
• Van Anh Do-Thi, Sang Min Park, Song Mi Park, Hye Jin Jeong, Geunyoung Cho, Hyun-Jung An et al. IL9 Polarizes Macrophages to M1 and Induces the Infiltration of Antitumor Immune Cells via MIP-1 and CXCR3 Chemokines Cancer Research Communications 1/18/2023 [PMID: 36968220]
• YC Huang, CY Chang, YY Wu, KL Wu, YM Tsai, HC Lee, EM Tsai, YL Hsu Single-Cell Transcriptomic Profiles of Lung Pre-Metastatic Niche Reveal Neutrophil and Lymphatic Endothelial Cell Roles in Breast Cancer Cancers, 2022-12-28;15(1):. 2022-12-28 [PMID: 36612175] (Flow Cytometry, Transgenic Mouse)
• F Chen, DW El-Naccach, JJ Ponessa, A Lemenze, V Espinosa, W Wu, K Lothstein, L Jin, O Antao, JS Weinstein, P Damani-Yok, K Khanna, PJ Murray, A Rivera, MC Siracusa, WC Gause Helminth resistance is mediated by differential activation of recruited monocyte-derived alveolar macrophages and arginine depletion Cell Reports, 2022-01-11;38(2):110215. 2022-01-11 [PMID: 35021079] (Flow Cytometry, Mouse)
• Tomasz M. Grzywa, Anna Sosnowska, Zuzanna Rydzynska, Michal Lazniewski, Dariusz Plewczynski, Klaudia Klicka et al. Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells Communications Biology 12/10/2021 [PMID: 34893694]
• Xi Tan, Cuo Yi, Yi Zhang, Najiao Tang, Yali Xu, Zheng Liu Ultrasound-Targeted Microbubble Destruction Alleviates Immunosuppression Induced by CD71+ Erythroid Progenitor Cells and Promotes PDL-1 Blockade Immunotherapy in the Lewis Lung Cancer Model Frontiers in Oncology 10/22/2021 [PMID: 34746009]
• Y Zhang, H Huang, M Coleman, A Ziemys, P Gopal, SM Kazmi, RA Brekken VEGFR2 activity on myeloid cells mediates immune suppression in the tumor microenvironment JCI Insight, 2021-12-08;6(23):. 2021-12-08 [PMID: 34673569] (Flow Cytometry, Mouse)
• J Xu, A Ganguly, J Zhao, M Ivey, R Lopez, JJ Osterholze, CS Cho, MA Olszewski CCR2 Signaling Promotes Brain Infiltration of Inflammatory Monocytes and Contributes to Neuropathology during Cryptococcal Meningoencephalitis MBio, 2021-07-27;0(0):e0107621. 2021-07-27 [PMID: 34311579] (Flow Cytometry, Mouse)
• Marques RM, Gonzalez-Nunez M, Walker ME et al. Loss of 15-lipoxygenase disrupts T(reg) differentiation altering their pro-resolving functions Cell Death & Differentiation 2021-11-01 [PMID: 34040168]
• Marques RM, Gonzalez-Nunez M, Walker ME et al. Loss of 15-lipoxygenase disrupts T(reg) differentiation altering their pro-resolving functions Cell Death & Differentiation 11/1/2021 [PMID: 34040168]
• Du W, Huang H, Wang Z et al. AXL is a key factor for cell plasticity and promotes metastasis in pancreatic cancer Mol Cancer Res 4/3/2021 [PMID: 33811159]
• SW Lee, HJ Park, J Jeon, YH Park, TC Kim, SH Jeon, RH Seong, L Van Kaer, S Hong Ubiquitous Overexpression of Chromatin Remodeling Factor SRG3 Exacerbates Atopic Dermatitis in NC/Nga Mice by Enhancing Th2 Immune Responses International Journal of Molecular Sciences, 2021-02-04;22(4):. 2021-02-04 [PMID: 33557054] (Flow Cytometry, Mouse)
• S Shakya, K Asosingh, JA Mack, EV Maytin Optimized protocol for the preparation of single cells from cutaneous wounds for flow cytometric cell sorting and analysis of macrophages MethodsX, 2020-08-08;7(0):101027. 2020-08-08 [PMID: 32939347] (Flow Cytometry, Mouse)
• W Miao, Y Zhao, Y Huang, D Chen, C Luo, W Su, Y Gao IL-13 Ameliorates Neuroinflammation and Promotes Functional Recovery after Traumatic Brain Injury J. Immunol., 2020-02-07;0(0):. 2020-02-07 [PMID: 32034062] (Flow Cytometry, Mouse)
• Maria Belen Garcia-Fabiani, Andrea Comba, Padma Kadiyala, Santiago Haase, Felipe Javier Núñez, David Altshuler et al. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system Methods in Enzymology 2/1/2020 [PMID: 32000905]
• Kara J. Filbey, Fumi Varyani, Yvonne Harcus, James P. Hewitson, Danielle J. Smyth, Henry J. McSorley et al. Macrophage Migration Inhibitory Factor (MIF) Is Essential for Type 2 Effector Cell Immunity to an Intestinal Helminth Parasite Frontiers in Immunology 10/24/2019 [PMID: 31708913]
• W Cai, X Dai, J Chen, J Zhao, M Xu, L Zhang, B Yang, W Zhang, M Rocha, T Nakao, J Kofler, Y Shi, RA Stetler, X Hu, J Chen STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice JCI Insight, 2019-10-17;4(20):. 2019-10-17 [PMID: 31619589] (Flow Cytometry, Mouse)
• A Carestia, HA Mena, CM Olexen, JM Ortiz Wilc, S Negrotto, AE Errasti, RM Gómez, CN Jenne, EA Carrera Si, M Schattner Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice Cell Rep, 2019-07-23;28(4):896-908.e5. 2019-07-23 [PMID: 31340152] (Flow Cytometry, Mouse)
• W Du, H Huang, N Sorrelle, RA Brekken Sitravatinib potentiates immune checkpoint blockade in refractory cancer models JCI Insight, 2018-11-02;3(21):. 2018-11-02 [PMID: 30385724] (Flow Cytometry, Mouse)
• KA Smith, S Löser, F Varyani, Y Harcus, HJ McSorley, AN McKenzie, RM Maizels Concerted IL-25R and IL-4R? signaling drive innate type 2 effector immunity for optimal helminth expulsion Elife, 2018-09-21;7(0):. 2018-09-21 [PMID: 30238872] (Flow Cytometry, Mouse)
• JE Tomlinson, E Žygelyt?, JK Grenier, MG Edwards, J Cheetham Temporal changes in macrophage phenotype after peripheral nerve injury J Neuroinflammation, 2018-06-15;15(1):185. 2018-06-15 [PMID: 29907154] (Flow Cytometry, Mouse)
• Pistorius K, Souza PR, De Matteis R et al. PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function Cell Chem Biol May 16 2018 [PMID: 29805036] (Flow) Cell Chem Biol. 5/24/2018 [PMID: 29805036]
  
  Details:
  Citation using the Alexa Fluor 647 form of this antibody.
• D Chen, J Xie, R Fiskesund, W Dong, X Liang, J Lv, X Jin, J Liu, S Mo, T Zhang, F Cheng, Y Zhou, H Zhang, K Tang, J Ma, Y Liu, B Huang Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype Nat Commun, 2018-02-28;9(1):873. 2018-02-28 [PMID: 29491374] (Flow Cytometry, Mouse)
• Evangelos Giampazolias, Barbara Zunino, Sandeep Dhayade, Florian Bock, Catherine Cloix, Kai Cao et al. Mitochondrial permeabilization engages NF-kappa B-dependent anti-tumour activity under caspase deficiency Nature Cell Biology 9/1/2017 [PMID: 28846096]
• BM Abidin, A Hammami, S Stäger, KM Heinonen Infection-adapted emergency hematopoiesis promotes visceral leishmaniasis PLoS Pathog., 2017-08-07;13(8):e1006422. 2017-08-07 [PMID: 28787450] (Flow Cytometry, Mouse)
• AE Shay, BT Diwakar, BJ Guan, V Narayan, JF Urban, KS Prabhu IL-4 upregulates cyclooxygenase-1 expression in macrophages J. Biol. Chem., 2017-07-06;0(0):. 2017-07-06 [PMID: 28684424] (Flow Cytometry, Mouse)
• NM Wilson, ME Gurney, WD Dietrich, CM Atkins Therapeutic benefits of phosphodiesterase 4B inhibition after traumatic brain injury PLoS ONE, 2017-05-19;12(5):e0178013. 2017-05-19 [PMID: 28542295] (Flow Cytometry, Mouse)
• JH Baek, GM Many, FJ Evesson, VR Kelley Dysferlinopathy Promotes an Intramuscle Expansion of Macrophages with a Cyto-Destructive Phenotype Am. J. Pathol., 2017-04-13;0(0):. 2017-04-13 [PMID: 28412297] (Flow Cytometry, Mouse)
• Jesmond Dalli, Romain A. Colas, Hildur Arnardottir, Charles N. Serhan Vagal Regulation of Group 3 Innate Lymphoid Cells and the Immunoresolvent PCTR1 Controls Infection Resolution Immunity 1/5/2017 [PMID: 28065837]
• Jesmond Dalli, Romain A. Colas, Hildur Arnardottir, Charles N. Serhan Vagal Regulation of Group 3 Innate Lymphoid Cells and the Immunoresolvent PCTR1 Controls Infection Resolution Immunity 2017-01-05 [PMID: 28065837] (Flow Cytometry, Mouse)
• Francesc Miró-Mur, Isabel Pérez-de-Puig, Maura Ferrer-Ferrer, Xabier Urra, Carles Justicia, Angel Chamorro et al. Immature monocytes recruited to the ischemic mouse brain differentiate into macrophages with features of alternative activation Brain, Behavior, and Immunity 2016-03-01 [PMID: 26275369] (Flow Cytometry, Transgenic Mouse)
• Baek J, Zeng R, Weinmann-Menke J, Valerius M, Wada Y, Ajay A, Colonna M, Kelley V IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease. J Clin Invest, 2015-06-29;125(8):3198-214. 2015-06-29 [PMID: 26121749] (Flow Cytometry, Mouse)
• Xian Jin, Tongqing Yao, Zhong’e Zhou, Jian Zhu, Song Zhang, Wei Hu et al. Advanced Glycation End Products Enhance Macrophages Polarization into M1 Phenotype through Activating RAGE/NF-kappa B Pathway BioMed Research International 5/31/2015 [PMID: 26114112]
• Liechtenstein T, Perez-Janices N, Blanco-Luquin I, Goyvaerts C, Schwarze J, Dufait I, Lanna A, Ridder M, Guerrero-Setas D, Breckpot K, Escors D Anti-melanoma vaccines engineered to simultaneously modulate cytokine priming and silence PD-L1 characterized using myeloid-derived suppressor cells as a readout of therapeutic efficacy. Oncoimmunology, 2014-07-03;3(7):e945378. 2014-07-03 [PMID: 25954597] (Flow Cytometry, Mouse)
• Hashimoto T, Satoh T, Yokozeki H Protective Role of STAT6 in Basophil-Dependent Prurigo-like Allergic Skin Inflammation. J Immunol, 2015-04-10;194(10):4631-40. 2015-04-10 [PMID: 25862819] (Flow Cytometry, Mouse)
• Hale , Malika, Itani , Farah, Buchta , Claire M, Wald , Gal, Bing , Megan, Norian , Lyse A Obesity triggers enhanced MDSC accumulation in murine renal tumors via elevated local production of CCL2. PLoS ONE, 2015-03-13;10(3):e0118784. 2015-03-13 [PMID: 25769110] (Flow Cytometry, Mouse)

Review for Arginase 1/ARG1/liver Arginase Antibody (IC5868P) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Nuria Álvarez-Sánchez Flow Mouse 02/21/2019

Summary

• Application: Flow Cytometry
• Sample Tested: brain and spinal cord
• Species: Mouse
• Lot: ADBB0617041

Comments

• Comments: Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for CD11b, CD19, Arg1, iNOS, IL-6, IL-10, IL-35, TNF and with a marker for dead cells to exclude them. Viable, CD11b+ cells were gated and Arg1 staining is showed in control cells, non-stained for Arg1 (upper plot), and in Arg1-stained cells (lower plot).

Bioinformatics

• Gene Symbol: ARG1
• Uniprot:
  • P05089()