Arginase 1/ARG1/liver Arginase Antibody [Alexa Fluor® 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

Arginase 1/ARG1/liver Arginase Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Arginase 1/ARG1
  Met1-Lys322
  Accession # P05089
• Specificity: Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Arginase 1/ARG1/liver Arginase Antibody [Alexa Fluor® 594]

• AI
• ARG1
• Arginase 1
• arginase, liver
• Arginase-1
• EC 3.5.3.1
• EC:3.5.3.1
• Liver Arginase
• Liver-type arginase
• PGIF
• Type I Arginase

Background

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.