Angiopoietin-like Protein 4/ANGPTL4 Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Angiopoietin-like Protein 4/ANGPTL4 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 4
  Leu165-Ser406
  Accession # Q9BY76
• Specificity: Detects human ANGPTL4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ANGPTL3, rhAngiopoietin-1, -2, -3, and -4 is observed.In Western blots, approximately 1% cross-reactivity with rhANGPLT4 N-terminal Fragment is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANGPTL4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Human Angiopoietin-like Protein 4/ANGPTL4 Antibody (Catalog # AF3485)
  ELISA Detection: Human Angiopoietin-like Protein 4/ANGPTL4 Biotinylated Antibody (Catalog # BAF3485)
  Standard: Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # 4487-AN)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

ANGPTL4, also known as PPAR gamma angiopoietin-related protein (PGAR), hepatic fibrinogen/angiopoietin-related protein (HFARP) and fasting-induced adipose factor (FIAF), is a secreted protein that shares structural homology with angiopoietins. It contains an N-terminal coiled-coil region that mediates covalent homo-oligomerization and a C-terminal fibrinogen-like domain. ANGPTL4 undergoes proteolytic processing and releases the C-terminal domain, which circulates as a monomer. At least 2 additional splice isoforms exist. ANGPTL4 is most highly expressed in adipose tissues. Its expression is up-regulated in endothelial cells and cardiomyocytes during hypoxia. ANGPTL4 is involved in the regulation of lipid and glucose metabolism. It has also been associated with angiogenesis. The amino acid sequence of human ANGPTL4 is 81%, 76% and 81% identical to that of porcine, mouse and canine ANGPTL4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANGPTL4