Neprilysin-2/MMEL1 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price

Neprilysin-2/MMEL1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neprilysin-2/MMEL1
Gly69-Trp770
Accession # AAL08942
Specificity
Detects human Neprilysin-2/MMEL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Neprilysin is observed and less than 1% cross-reactivity with rhECE-1 and rhECE-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMEL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 4
using
AF2340 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neprilysin-2/MMEL1 Antibody

  • EC 3.4.24
  • EC 3.4.24.11
  • MELL1
  • membrane metallo-endopeptidase-like 1
  • Membrane metallo-endopeptidase-like 2MGC119455
  • MGC119454
  • MMEL1
  • MMEL2
  • NEP2
  • NEP2(m)
  • NEPIIMGC119456
  • Neprilysin II
  • Neprilysin2
  • Neprilysin-2
  • NL2
  • NL2NL1
  • SEP
  • soluble secreted endopeptidase
  • zinc metallopeptidase

Background

Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles. The ectodomain of human Neprilysin-2/MMEL1 was expressed with an N-terminal His tag and purified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neprilysin-2/MMEL1 Antibody (AF2340) (0)

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Review for Neprilysin-2/MMEL1 Antibody (AF2340) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2340:
Filter by Applications
WB
(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/06/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22008264
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22008264

Details

Detection NotesSee PMID 22008264

Comments

CommentsDetection of overexpressed MMEL1/2 (NEP2).Published: See PMID 22008264

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Bioinformatics Tool for Neprilysin-2/MMEL1 Antibody (AF2340)

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Diseases for Neprilysin-2/MMEL1 Antibody (AF2340)

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Anonymous
01/06/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol MMEL1
Entrez
Uniprot