Human Angiopoietin-like 3 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Angiopoietin-like 3 Quantikine ELISA Kit Summary

• Background: The Quantikine Human ANGPTL3 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human ANGPTL3 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human ANGPTL3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ANGPTL3 showed linear curves that were parallel to the standard curves o...btained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ANGPTL3.
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• Specificity: Natural and recombinant human ANGPTL3
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ANGPTL3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Angiopoietin-like 3 Quantikine ELISA Kit

• AGNPT5
• Ang-5
• angiopoietin 5
• Angiopoietin-5
• angiopoietin-like 3
• Angiopoietin-like Protein 3
• angiopoietin-related protein 3
• ANGPT5angiopoietin-5
• ANGPTL3
• FHBL2

Background

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Angiopoietin-like Protein 3/ANGPTL3 (DANL30)(8)

Publications using DANL30

• Watts, GF;Schwabe, C;Scott, R;Gladding, PA;Sullivan, D;Baker, J;Clifton, P;Hamilton, J;Given, B;Melquist, S;Zhou, R;Chang, T;San Martin, J;Gaudet, D;Goldberg, IJ;Knowles, JW;Hegele, RA;Ballantyne, CM; RNA interference targeting ANGPTL3 for triglyceride and cholesterol lowering: phase 1 basket trial cohorts Nature medicine 2023-08-25 [PMID: 37626170] ( Human)
• M Subramania, J McIninch, I Zlatev, MK Schlegel, C Kaittanis, T Nguyen, S Agarwal, T Racie, MA Alvarado, K Wassarman, TS Collins, T Chickering, CR Brown, K Schmidt, AB Castoreno, S Shulga-Mor, E Stamenova, K Buckowing, D Berman, JD Barry, A Bisbe, MA Maier, K Fitzgerald, V Jadhav RNAi-mediated rheostat for dynamic control of AAV-delivered transgenes Nature Communications, 2023-04-08;14(1):1970. 2023-04-08 [PMID: 37031257] (Human)
• MR Taskinen, E Björnson, N Matikainen, S Söderlund, J Rämo, MM Ainola, A Hakkaraine, C Sihlbom, A Thorsell, L Andersson, PO Bergh, M Henricsson, S Romeo, M Adiels, S Ripatti, M Laakso, CJ Packard, J Borén Postprandial metabolism of apolipoproteins B48, B100, C-III and E in humans with APOC3 loss-of-function mutations JCI Insight, 2022-10-10;0(0):. 2022-10-10 [PMID: 36040803] (Human)
• M Qaddoumi, P Hebbar, M Abu-Farha, A Al Somaly, M Melhem, F Al-Kayal, I AlKhairi, P Cherian, M Alanbaei, F Al-Mulla, J Abubaker, TA Thanaraj GALNT2 rs4846914 SNP Is Associated with Obesity, Atherogenic Lipid Traits, and ANGPTL3 Plasma Level Genes, 2022-07-04;13(7):. 2022-07-04 [PMID: 35885984] (Human)
• M Alanbaei, M Abu-Farha, P Hebbar, M Melhem, BS Chandy, E Anoop, P Cherian, I Al-Khairi, F Alkayal, F Al-Mulla, J Abubaker, TA Thanaraj ANGPTL3 Variants Associate with Lower Levels of Irisin and C-Peptide in a Cohort of Arab Individuals Genes, 2021-05-17;12(5):. 2021-05-17 [PMID: 34067751] (Human)
• MF Garcés, JD Buell-Acos, HA Rodríguez-, E Pulido-Sán, JJ Rincon-Ram, DC Moreno-Ord, R Franco-Veg, JS Roncancio-, AJ Burgos-Car, E Lacunza, JP Castaño, C Diéguez, R Nogueiras, E Sanchez, JE Caminos Serum angiopoietin-like 3 levels are elevated in obese non diabetic men but are unaffected during an oral glucose tolerance test Scientific Reports, 2020-12-03;10(1):21118. 2020-12-03 [PMID: 33273510] (Human)
• P Ku?, R Charkiewic, D Klasa-Mazu, T Milczek, B Mroczko, J Nikli?ski, P Lauda?ski Profiling of selected angiogenesis-related genes in serous ovarian cancer patients Adv Med Sci, 2017-02-21;62(1):116-120. 2017-02-21 [PMID: 28235714] (Human)
• Chung H, Lee M, Hwang S, Lee H, Yoo H, Seo J, Kim S, Kim N, Baik S, Choi D, Kim S, Choi K Circulating angiopoietin-like protein 8 (ANGPTL8) and ANGPTL3 concentrations in relation to anthropometric and metabolic profiles in Korean children: a prospective cohort study. Cardiovasc Diabetol, 2016-01-06;15(1):1. 2016-01-06 [PMID: 26739706] (Human)

FAQs for Angiopoietin-like Protein 3/ANGPTL3 (DANL30). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ANGPTL3