Neuroligin 1/NLGN1 Antibody (S97A-31) - BSA Free

Western Blot: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Western Blot analysis of Mouse Brain Membrane showing detection of ~120 kDa Neuroligin 1/NLGN1 protein using Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody, Clone S97A-31 (NBP2-42192). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody (NBP2-42192) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~120 kDa.

Immunocytochemistry/Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody, Clone S97A-31 (NBP2-42192). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody (NBP2-42192) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 1/NLGN1 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.

Immunocytochemistry/Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody, Clone S97A-31 (NBP2-42192). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody (NBP2-42192) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 1/NLGN1 Antibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S97A-31
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Neuroligin 1/NLGN1 Antibody (S97A-31) - BSA Free Summary

• Description: Novus Biologicals Mouse Neuroligin 1/NLGN1 Antibody (S97A-31) - BSA Free (NBP2-42192) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Neuroligin 1/NLGN1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1. Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) >40% identity with Neuroligin-2 and -3.
• Localization: Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density
• Specificity: Greater then 40% identity with Neuroligin-2 and -3.Detects approx 120kDa. Does not cross-react with other Neuroligins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NLGN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 1:1000
• Application Notes: 2 ug/ml of Neuroligin 1 Antibody was sufficient for detection of Neuroligin-1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Neuroligin 1/NLGN1 Antibody (S97A-31) - BSA Free

• EC 3.1.1
• EC 3.1.1.1
• KIAA1070neuroligin-1
• MGC45115
• Neuroligin 1
• NL1
• Nlg
• NLGN1

Background

Neuroligin 1 encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuroligin 1/NLGN1 Antibody (NBP2-42192)(1)

Publication using NBP2-42192

• Hacisuleyman E, Hale CR, Noble N et Al. Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding Nat Neurosci 2024-04-08 [PMID: 38589584]

Bioinformatics

• Gene Symbol: NLGN1
• Uniprot:
  • Q62765()