Simple Western lane view shows lysates of Human Diabetic Adipose, loaded at 0.2 mg/mL. A specific band was detected for Angiopoietin‑like Protein 4/ANGPTL4 at approximately 59 kDa (as indicated) using 50 ...read more
Angiopoietin-like Protein 4/ANGPTL4 Antibody [Unconjugated] Summary
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 4/ANGPLT4 Leu165-Ser406 Accession # Q9BY76
Detects human ANGPTL4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ANGPTL3, rhAngiopoietin-1, -2, -3, and -4 is observed. In Western blots, approximately 1% cross-reactivity with rhANGPLT4 N-terminal Fragment is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
ELISA Capture: Human Angiopoietin-like Protein 4/ANGPTL4 Antibody (Catalog # AF3485) ELISA Detection: Human Angiopoietin-like Protein 4/ANGPTL4 Biotinylated Antibody (Catalog # BAF3485) Standard: Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # 4487-AN)
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Angiopoietin-like Protein 4/ANGPTL4 Antibody [Unconjugated]
Angiopoietin like Protein 4
Angiopoietin-like Protein 4
ARP4fasting-induced adipose factor
FIAFhepatic angiopoietin-related protein
Hepatic fibrinogen/angiopoietin-related protein
peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein
PGARangiopoietin-related protein 4
PPARG angiopoietin related protein
ANGPTL4, also known as PPAR gamma angiopoietin-related protein (PGAR), hepatic fibrinogen/angiopoietin-related protein (HFARP) and fasting-induced adipose factor (FIAF), is a secreted protein that shares structural homology with angiopoietins. It contains an N-terminal coiled-coil region that mediates covalent homo-oligomerization and a C-terminal fibrinogen-like domain. ANGPTL4 undergoes proteolytic processing and releases the C-terminal domain, which circulates as a monomer. At least 2 additional splice isoforms exist. ANGPTL4 is most highly expressed in adipose tissues. Its expression is up-regulated in endothelial cells and cardiomyocytes during hypoxia. ANGPTL4 is involved in the regulation of lipid and glucose metabolism. It has also been associated with angiogenesis. The amino acid sequence of human ANGPTL4 is 81%, 76% and 81% identical to that of porcine, mouse and canine ANGPTL4, respectively.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Angiopoietin-like Protein 4/ANGPTL4 Antibody [Unconjugated] and receive a gift card or discount.