Angiopoietin-like Protein 4/ANGPTL4 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of Human Diabetic Adipose, loaded at 0.2 mg/mL. A specific band was detected for Angiopoietin‑like Protein 4/ANGPTL4 at approximately 59 kDa (as indicated) using 50 ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Simple Western, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Angiopoietin-like Protein 4/ANGPTL4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 4/ANGPLT4
Leu165-Ser406
Accession # Q9BY76
Specificity
Detects human ANGPTL4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ANGPTL3, rhAngiopoietin-1, -2, -3, and -4 is observed. In Western blots, approximately 1% cross-reactivity with rhANGPLT4 N-terminal Fragment is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANGPTL4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Simple Western 50 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human Angiopoietin-like Protein 4/ANGPTL4 Antibody (Catalog # AF3485)
ELISA Detection: Human Angiopoietin-like Protein 4/ANGPTL4 Biotinylated Antibody (Catalog # BAF3485)
Standard: Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # 4487-AN)
Publications
Read Publications using
AF3485 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 4/ANGPTL4 Antibody [Unconjugated]

  • Angiopoietin like Protein 4
  • angiopoietin-like 4
  • Angiopoietin-like Protein 4
  • ANGPTL4
  • ARP4fasting-induced adipose factor
  • FIAF
  • FIAFhepatic angiopoietin-related protein
  • Hepatic fibrinogen/angiopoietin-related protein
  • HFARP
  • HFARPANGPTL2
  • NL2
  • peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein
  • PGAR
  • PGARangiopoietin-related protein 4
  • pp1158
  • PPARG angiopoietin related protein

Background

ANGPTL4, also known as PPAR gamma angiopoietin-related protein (PGAR), hepatic fibrinogen/angiopoietin-related protein (HFARP) and fasting-induced adipose factor (FIAF), is a secreted protein that shares structural homology with angiopoietins. It contains an N-terminal coiled-coil region that mediates covalent homo-oligomerization and a C-terminal fibrinogen-like domain. ANGPTL4 undergoes proteolytic processing and releases the C-terminal domain, which circulates as a monomer. At least 2 additional splice isoforms exist. ANGPTL4 is most highly expressed in adipose tissues. Its expression is up-regulated in endothelial cells and cardiomyocytes during hypoxia. ANGPTL4 is involved in the regulation of lipid and glucose metabolism. It has also been associated with angiogenesis. The amino acid sequence of human ANGPTL4 is 81%, 76% and 81% identical to that of porcine, mouse and canine ANGPTL4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-like Protein 4/ANGPTL4 Antibody (AF3485)(15)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 5 applications: ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), Western Blot.


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ELISA Development
(1)
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(2)
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Human
(9)
Rat
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF3485 Applications Species
Y Zuo, L Dai, L Li, Y Huang, X Liu, X Liu, X Duan, S Jiang, GM Deng, H Chen ANGPTL4 Regulates Psoriasis via Modulating Hyperproliferation and Inflammation of Keratinocytes Frontiers in Pharmacology, 2022-07-04;13(0):850967. 2022-07-04 [PMID: 35860030] (Western Blot, Human) Western Blot Human
Yan Q. Chen, Thomas G. Pottanat, Robert W. Siegel, Mariam Ehsani, Yue-Wei Qian, Eugene Y. Zhen et al. Angiopoietin-like protein 8 differentially regulates ANGPTL3 and ANGPTL4 during postprandial partitioning of fatty acids[S] Journal of Lipid Research 8/1/2020 [PMID: 32487544]
YT Kang, WC Hsu, CC Ou, HC Tai, HT Hsu, KT Yeh, JL Ko Metformin Mitigates Nickel-Elicited Angiopoietin-Like Protein 4 Expression via HIF-1&alpha for Lung Tumorigenesis Int J Mol Sci, 2020-01-17;21(2):. 2020-01-17 [PMID: 31963541] (Western Blot, Human) Western Blot Human
BW van der Ko, RG Vink, JWE Jocken, NJT Roumans, GH Goossens, ECM Mariman, MA van Baak, EE Blaak Effect of diet-induced weight loss on angiopoietin-like protein 4 and adipose tissue lipid metabolism in overweight and obese humans Physiol Rep, 2018-07-01;6(13):e13735. 2018-07-01 [PMID: 29998530] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
EG Gruppen, S Kersten, RPF Dullaart Plasma angiopoietin-like 4 is related to phospholipid transfer protein activity in diabetic and non-diabetic subjects: role of enhanced low grade inflammation Lipids Health Dis, 2018-03-27;17(1):60. 2018-03-27 [PMID: 29587751] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
W Dijk, S Schutte, EO Aarts, IMC Janssen, L Afman, S Kersten Regulation of angiopoietin-like 4 and lipoprotein lipase in human adipose tissue J Clin Lipidol, 2018-02-17;0(0):. 2018-02-17 [PMID: 29555209] (ELISA Capture, Human) ELISA Capture Human
Jin-Wu Chen, Ying-Jia Luo, Zheng-Fei Yang, Li-Qiang Wen, Lin Huang Knockdown of angiopoietin-like 4 inhibits the development of human gastric cancer Oncology Reports 2/7/2018 [PMID: 29436683]
L Zhou, Y Huang, H Xie, X Mei Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels Mol Med Rep, 2017-12-19;0(0):. 2017-12-19 [PMID: 29257347] (Western Blot, Rat) Western Blot Rat
S Izraely, S Ben-Menach, O Sagi-Assif, T Meshel, DM Marzese, S Ohe, I Zubrilov, M Pasmanik-C, DSB Hoon, IP Witz ANGPTL4 promotes the progression of cutaneous melanoma to brain metastasis Oncotarget, 2017-07-05;8(44):75778-75796. 2017-07-05 [PMID: 29100268] (Western Blot, Human) Western Blot Human
K Baba, Y Kitajima, S Miyake, J Nakamura, K Wakiyama, H Sato, K Okuyama, H Kitagawa, T Tanaka, M Hiraki, K Yanagihara, H Noshiro Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines Sci Rep, 2017-09-11;7(1):11127. 2017-09-11 [PMID: 28894280] (Western Blot, Human) Western Blot Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol ANGPTL4
Uniprot