Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Unconjugated]

Images

 
Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed ...read more
Angiopoietin‑like Protein 6/ANGPTL6 was detected in immersion fixed BaF3 mouse pro-B cell line using Rat Anti-Mouse Angiopoietin‑like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB7527) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clone
766544
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Angiopoietin-like Protein 6/ANGPTL6
Ala25-Leu457
Accession # Q8ROZ6
Specificity
Detects mouse Angiopoietin-like Protein 6/ANGPTL6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Angiopoietin-like 6 or recombinant mouse Angiopoietin-like 2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ANGPTL6
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Unconjugated]

  • AGF
  • AGFAngiopoietin-related protein 5
  • Angiopoietin like Protein 6
  • angiopoietin-like 6
  • Angiopoietin-like Protein 6
  • Angiopoietin-related growth factor
  • ANGPTL6
  • ARP5
  • ARP5angiopoietin-related protein 6

Background

Angiopoietin-like 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain (aa 51-77,
126-164) and a fibrinogen-like domain (aa 238-456). A conserved Integrin-binding RGD motif is located within the fibrinogen-like domain. This enables ANGPTL6 to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. ANGPTL6 also promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. ANGPTL6 is also secreted by hepatocytes. It inhibits gluconeogenesis in these cells and promotes insulin sensitivity and energy expenditure in mice fed high fat diets. Serum levels of ANGPTL6 are elevated in metabolic syndrome, diabetes, and preeclampsia but are decreased in chronic renal failure. ANGPTL6 is additionally produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature mouse ANGPTL6 shares 75% and 95% sequence identity with human and rat ANGPTL6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3485
Species: Hu, Pm
Applications: WB, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF313
Species: Hu
Applications: WB, Simple Western, IHC, Block
AF619
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready
AF1444
Species: Mu
Applications: WB, IHC
NBP1-78758
Species: Hu
Applications: WB (-), IP
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
NBP1-80533
Species: Hu, Mu
Applications: WB
NB300-605
Species: Hu, Mu, Rt, Po
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro
NB100-61628
Species: Hu, Mu
Applications: IP (-), WB, Simple Western, ICC/IF, IHC, IHC-P
H00057180-B01P
Species: Hu
Applications: WB
AF2288
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NBP2-82073
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527) (0)

There are no publications for Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527) (0)

There are no reviews for Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Angiopoietin-like Protein 6/ANGPTL6 Products

Bioinformatics Tool for Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527)

Discover related pathways, diseases and genes to Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527)

Discover more about diseases related to Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527).
 

Pathways for Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527)

View related products by pathway.

PTMs for Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527)

Learn more about PTMs related to Angiopoietin-like Protein 6/ANGPTL6 Antibody (MAB7527).

Blogs on Angiopoietin-like Protein 6/ANGPTL6

There are no specific blogs for Angiopoietin-like Protein 6/ANGPTL6, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol ANGPTL6
Uniprot