Angiopoietin-like Protein 3/ANGPTL3 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - Human hepatoma, using ANGPTL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - HepG2 whole cell extract and conditioned medium (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin-like Protein 3/ANGPTL3 antibody (NBP2-15390) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - Angiopoietin-like Protein 3/ANGPTL3 antibody detects Angiopoietin-like Protein 3/ANGPTL3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human hepatocellular carcinoma.Angiopoietin-like Protein 3/ANGPTL3 stained by Angiopoietin-like Protein 3/ANGPTL3 antibody (NBP2-15390) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Angiopoietin-like Protein 3/ANGPTL3 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Angiopoietin-like Protein 3/ANGPTL3. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANGPTL3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (80%), Rat (80%), Pig (85%), Bovine (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Angiopoietin-like Protein 3/ANGPTL3 Antibody - Azide and BSA Free

• AGNPT5
• Ang-5
• angiopoietin 5
• Angiopoietin-5
• angiopoietin-like 3
• Angiopoietin-like Protein 3
• angiopoietin-related protein 3
• ANGPT5angiopoietin-5
• ANGPTL3
• FHBL2

Background

The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANGPTL3
• Uniprot:
  • Q9Y5C1(Human)