3829-AN) was serially diluted and captured by Rat Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3829) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Angiopoietin‑like Protein 3/ANGPTL3 (Catalog # 3829-AN) was serially diluted and captured by Rat Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3829) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3829-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89087-25ul | |||
136-AN) was serially diluted and captured by Rat Anti-Mouse Angiopoietin‑like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB13621) coated on a Clear Polystyrene Microplate (Catalog # DY990). Detection antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse Angiopoietin‑like Protein 3/ANGPTL3 (Catalog # 136-AN) was serially diluted and captured by Rat Anti-Mouse Angiopoietin‑like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB13621) coated on a Clear Polystyrene Microplate (Catalog # DY990). Detection antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB13621-SP | |||
3829-AN) was serially diluted and captured by Rat Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3829) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Angiopoietin‑like Protein 3/ANGPTL3 (Catalog # 3829-AN) was serially diluted and captured by Rat Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3829) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB38291-SP | |||
HAF005). A specific band was detected for Angiopoietin-like Protein 3/ANGPTL3 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Raji human Burkitt's lymphoma cell line (negative control), HepG2 human hepatocellular carcinoma cell line, and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Angiopoietin-like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38292) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-like Protein 3/ANGPTL3 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB38292-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB136-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1362-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3829-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60244-50ul | |||
![Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP1-89087] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-Western-Blot-NBP1-89087-img0005.jpg "Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP1-89087] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP1-89087] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89087-img0008.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP1-89087] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89087-img0006.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP1-89087] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89087-img0007.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP1-89087] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-Immunohistochemistry-Paraffin-NBP1-89087-img0009.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [NBP3-16596] - Immunohistochemistry of paraffin-embedded mouse kidney using Angiopoietin-like Protein 3/ANGPTL3 Rabbit mAb (NBP3-16596) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-4W9A7-Immunohistochemistry-Paraffin-NBP3-16596-img0001.jpg "Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [NBP3-16596] - Immunohistochemistry of paraffin-embedded mouse kidney using Angiopoietin-like Protein 3/ANGPTL3 Rabbit mAb (NBP3-16596) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [NBP3-16596] - Confocal imaging of paraffin-embedded Rat liver using ANGPTL3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16596_rabbit-angiopoietin-like-protein-3-angptl3-mab-4w9a7-immunohistochemistry-paraffin-198202485115..jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [NBP3-16596] - Confocal imaging of paraffin-embedded Mouse liver using ANGPTL3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16596_rabbit-angiopoietin-like-protein-3-angptl3-mab-4w9a7-immunohistochemistry-paraffin-198202485220..jpg)
![Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [Angiopoietin-like Protein 3/ANGPTL3] - Western blot analysis of various lysates using Angiopoietin-like Protein 3/ANGPTL3 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16596_rabbit-angiopoietin-like-protein-3-angptl3-mab-4w9a7-52202517213530.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - Human hepatoma, using ANGPTL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ANGPTL3-Antibody-Immunohistochemistry-Paraffin-NBP2-15390-img0001.jpg "Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - Human hepatoma, using ANGPTL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - HepG2 whole cell extract and conditioned medium (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin-like Protein 3/ANGPTL3 antibody (NBP2-15390) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15390_rabbit-polyclonal-angiopoietin-like-protein-3-angptl3-antibody-91020242013125.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - Angiopoietin-like Protein 3/ANGPTL3 antibody detects Angiopoietin-like Protein 3/ANGPTL3 protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded human hepatocellular carcinoma.</br>Angiopoietin-like Protein 3/ANGPTL3 stained by Angiopoietin-like Protein 3/ANGPTL3 antibody (NBP2-15390) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15390_rabbit-polyclonal-angiopoietin-like-protein-3-angptl3-antibody-91020242063321.jpg)
![Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01] - Analysis of ANGPTL3 expression in transfected 293T cell line by ANGPTL3 monoclonal antibody (M01), clone 3B7.Lane 1: ANGPTL3 transfected lysate (Predicted MW: 53.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-3B7-Western-Blot-H00027329-M01-img0007.jpg "Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01] - Analysis of ANGPTL3 expression in transfected 293T cell line by ANGPTL3 monoclonal antibody (M01), clone 3B7.Lane 1: ANGPTL3 transfected lysate (Predicted MW: 53.6 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01] - Analysis of monoclonal antibody to ANGPTL3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-3B7-Immunohistochemistry-Paraffin-H00027329-M01-img0005.jpg)
![ELISA: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01] - Detection limit for recombinant GST tagged ANGPTL3 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-3B7-ELISA-H00027329-M01-img0008.jpg)
![Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) [NBP2-60244] - The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Angiopoietin-like Protein 3/ANGPTL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse kidney tissue lysate](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-23D8-Western-Blot-NBP2-60244-img0001.jpg "Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) [NBP2-60244] - The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Angiopoietin-like Protein 3/ANGPTL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse kidney tissue lysate")
![Immunocytochemistry/Immunofluorescence: Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) [NBP2-60244] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-23D8-Immunocytochemistry-Immunofluorescence-NBP2-60244-img0002.jpg)
