Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with ...read more
Polypeptide GalNac Transferase 2/GALNT2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Polypeptide GalNac Transferase 2/GALNT2 Antigen Affinity-purified ...read more
GALNT2 (N-Acetyl-Galactoseaminyl Transferase 2; also UDP-Acetylgalactosaminyltransferase 2 and ppGalNAc-T2) is a 68-74 kDa member of the GalNAC transferase subfamily, glycosyltransferase 2 family of enzymes. It is widely expressed, being found on basal keratinocytes, hepatocytes, B cells, renal tubular epithelium, and virtually all cell lines examined to date. GALNT2 is found in the Golgi apparatus, and catalyzes the transfer of UDP-GalNAC onto either a Ser or Thr residue on a previously glycosylated peptide/polypeptide backbone. The generation of O-linked carbohydrates is believed to play a role in cytokine proteolytic processing, as the presence of O-linked sugar adjacent to a PC processing site is known to inhibit proteolysis and molecule inactivation. Human GALNT2 is a 571 amino acid (aa) type II transmembrane protein. It contains a six aa N-terminal cytoplasmic region and a 547 aa extracellular domain (aa 25-571). The ECD possesses two key parts, a catalytic region with two catalytic subdomains (aa 135-240 and 300-362), and a ricin-type lectin domain that binds carbohydrates (aa 456‑566). The latter domain is suggested to facilitate GALNT2 action by imparting specificity and stability to the overall enzyme activity. A 52 kDa soluble form of GALNT2 has been reported that begins at Lys52. There are two potential splice form variants. Both contain a four aa substitution for aa 1-42, and one contains an additional four aa substitution for aa 543-571. Over aa 52-571, human GALNT2 shares 97% aa sequence identity with mouse GALNT2.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.