![Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.](http://images.novusbio.com/fullsize2/Apolipoprotein-B-ApoB-Antibody-Western-Blot-NB200-527-img0001.jpg "Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Mouse low density lipoprotein (LDL) |
| Localization | Secreted |
| Specificity | Apo B48 and B100. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | APOB |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -70C. Avoid freeze-thaw cycles. |
| Buffer | Whole antisera |
| Preservative | 0.02% Sodium Azide |
| Purity | Unpurified |
| Publication using NB200-527 | Applications | Species |
|---|---|---|
| Tsai WC, Hsu SD, Hsu CS et al. MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis J Clin Invest 2012 Aug 1 [PMID: 22820290] (WB, Mouse) | WB | Mouse |
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reviewed by:
Anonymous |
WB | Mouse | 03/01/2018 |
Summary
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reviewed by:
Anonymous |
WB | Mouse | 04/05/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for Apolipoprotein B/ApoB Antibody (NB200-527)Discover more about diseases related to Apolipoprotein B/ApoB Antibody (NB200-527).
| Pathways for Apolipoprotein B/ApoB Antibody (NB200-527)View related products by pathway.
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| Anonymous 03/01/2018 |
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| Application: | WB | |
| Species: | Mouse |
| Anonymous 04/05/2017 |
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| Application: | WB | |
| Species: | Mouse |
![Western Blot: LDL R Antibody (C7) [NBP1-78159] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-LDL receptor in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/LDL-R-Antibody-(C7)-Western-Blot-NBP1-78159-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2.](http://images.novusbio.com/fullsize2/LDL-R-Antibody-(C7)-Immunocytochemistry-Immunofluorescence-NBP1-78159-img0007.jpg)
![Immunohistochemistry: LDL R Antibody (C7) [NBP1-78159] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining.](http://images.novusbio.com/fullsize2/LDL-R-Antibody-(C7)-Immunohistochemistry-NBP1-78159-img0006.jpg)
![Western Blot: Lipoprotein a Antibody (4H1) [NBP2-37477] - Western blot analysis using LPA mAb against human LPA recombinant protein. (Expected MW is 34.1 kDa)](http://images.novusbio.com/fullsize2/Lipoprotein-a-Antibody-(4H1)-Western-Blot-NBP2-37477-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunofluorescence analysis of HepG2 cells using LPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/Lipoprotein-a-Antibody-(4H1)-Immunocytochemistry-Immunofluorescence-NBP2-37477-img0003.jpg)
![Immunohistochemistry: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using LPA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Lipoprotein-a-Antibody-(4H1)-Immunohistochemistry-NBP2-37477-img0006.jpg)
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Total protein from HepG2 and Hek293 cells was separated on a 4-15% gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-APOA1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NBP2-52979-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-APOA1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Apolipoprotein-A-I-ApoA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52979-img0001.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse liver using APOA1 antibody at 1:400 dilution. The antibody generated a weak/mild cytoplasmic signal in hepatocytes with a very strong positivity near the hepatocyte membranes as well as in the inter-cellular spaces.](http://images.novusbio.com/fullsize2/Apolipoprotein-A-I-ApoA1-Antibody-Immunohistochemistry-Paraffin-NBP2-52979-img0002.jpg)
![Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/Thyroglobulin-Antibody-(2H11+6E1)-Immunohistochemistry-Paraffin-NBP2-34294-img0005.jpg)
![Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).](http://images.novusbio.com/fullsize2/Thyroglobulin-Antibody-(2H11+6E1)-Immunohistochemistry-Paraffin-NBP2-34294-img0003.jpg)
![Immunohistochemistry: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/Thyroglobulin-Antibody-(2H11+6E1)-Immunohistochemistry-NBP2-34294-img0004.jpg)
![Western Blot: MTTP Antibody [NBP1-62489] - HepG2 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize2/MTTP-Antibody-Western-Blot-NBP1-62489-img0003.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize2/Apolipoprotein-E-ApoE-Antibody-(WUE-4)-Immunohistochemistry-Paraffin-NB110-60531-img0011.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain](http://images.novusbio.com/fullsize2/Apolipoprotein-E-ApoE-Antibody-(WUE-4)-Western-Blot-NB110-60531-img0013.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize2/Apolipoprotein-E-ApoE-Antibody-(WUE-4)-Flow-Cytometry-NB110-60531-img0014.jpg)
![SDS-Page: Recombinant Human Soluble Liver/Pancreas Antigen Protein [NBP1-50890]](http://images.novusbio.com/fullsize/Soluble-Liver-Pancreas-Antigen-Protein-SDS-Page-NBP1-50890-img0001.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]](http://images.novusbio.com/fullsize2/Insulin Antibody (E2-E3 (same as INS04))-SDS-Page-NBP2-34260-img0003.jpg)
![Western Blot: PCSK9 Antibody [NBP2-31364] - Western blot analysis of human lung tissue (A) using PCSK9 antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize2/PCSK9-Antibody-Western-Blot-NBP2-31364-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: PCSK9 Antibody [NBP2-31364] - PCSK9 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A concentration of 0.1 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize2/PCSK9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31364-img0012.jpg)
![Immunohistochemistry-Paraffin: PCSK9 Antibody [NBP2-31364] - IHC-P analysis of PCSK9 protein in a section of normal skin from human using 5 ug/ml concentration of PCSK9 antibody. The keratinocytes showed an expected cytoplasmic staining pattern with more intensity towards the cellular periphery, whereas the pigmented basel cell layer depicted relatively milder staining.](http://images.novusbio.com/fullsize2/PCSK9-Antibody-Immunohistochemistry-Paraffin-NBP2-31364-img0004.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NB600-610] - Tissue: liver.](http://images.novusbio.com/fullsize2/Apolipoprotein-CIII-Antibody-Immunohistochemistry-Paraffin-NB600-610-img0001.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Western Blot: LIPC Overexpression Lysate (Adult Normal) [NBL1-12547] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LIPC.](http://images.novusbio.com/fullsize/LIPC Overexpression Lysate (Adult Normal)-Western Blot-NBL1-12547-img0002.jpg)
![Western Blot: CETP Antibody [NBP1-45478] - Alcohol feeding showed no significant effect on the expression of CETP in mouse liver cells. Image from verified customer review.](http://images.novusbio.com/fullsize2/CETP-Antibody-Western-Blot-NBP1-45478-img0005.jpg)
![Western Blot: CETP Antibody [NBP1-45478] - Analysis of CETP expression in lysate from mouse small intestine (Lane 1) and Jurkat cells (Lane 2).](http://images.novusbio.com/fullsize2/CETP-Antibody-Western-Blot-NBP1-45478-img0004.jpg)
![Western Blot: CETP Antibody [NBP1-45478] - Jurkat cell lysate (60 ug).](http://images.novusbio.com/fullsize2/CETP-Antibody-Western-Blot-NBP1-45478-img0003.jpg)
![Western Blot: Metallothionein-1B Overexpression Lysate (Adult Normal) [NBP2-10437] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Metallothionein-1B.](http://images.novusbio.com/fullsize/Metallothionein-1B Overexpression Lysate (Adult Normal)-Western Blot-NBP2-10437-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0005.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.")