Apolipoprotein B/ApoB Antibody

Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Apolipoprotein B/ApoB Antibody Summary

• Immunogen: Mouse low density lipoprotein (LDL)
• Localization: Secreted
• Specificity: Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APOB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • Immunoprecipitation
• Application Notes: Use in immunoprecipitation reported in scientific literature (PMID:31807238).

Reactivity Notes

Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved. Rat reactivity reported in scientific literature (PMID: 29964320).

Packaging, Storage & Formulations

• Storage: Store at -70C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for Apolipoprotein B/ApoB Antibody

• Apo B-100
• APOB
• ApoB-100
• ApoB-48
• apolipoprotein B (including Ag(x) antigen)
• Apolipoprotein B
• apolipoprotein B-100
• apolipoprotein B48
• FLDB
• LDLCQ4
• mutant Apo B 100

Background

Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein B/ApoB Antibody (NB200-527)(5)

Publications using NB200-527

• Song Y, Kim S, Lee H et al. Chromenopyrimidinone Controls Stemness and Malignancy by suppressing CD133 Expression in Hepatocellular Carcinoma Cancers (Basel) May 8 2020 [PMID: 32397206] (WB, Human)
• Matsumoto A, Takahashi Y, Chang H et al. Blood concentrations of small extracellular vesicles are determined by a balance between abundant secretion and rapid clearance J Extracell Vesicles Jan 1 2019 [PMID: 31807238] (IP, Mouse)
• Howell GE, McDevitt E, Henein L et al. Alterations in cellular lipid metabolism produce neutral lipid accumulation following exposure to the organochlorine compound trans-nonachlor in rat primary hepatocytes. Environ. Toxicol. Jul 2 2018 [PMID: 29964320] (WB, Rat)
• Grefhorst A, van den Beukel JC, Dijk W et al. Multiple effects of cold exposure on livers of male mice J. Endocrinol. May 9 2018 [PMID: 29743343] (WB, Mouse)
• Tsai WC, Hsu SD, Hsu CS et al. MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis J Clin Invest 2012 Aug 1 [PMID: 22820290] (WB, Mouse)

Reviews for Apolipoprotein B/ApoB Antibody (NB200-527) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 03/01/2018

Summary

• Application: Western Blot
• Sample Tested: Liver
• Species: Mouse
• Lot: 2c08910

reviewed by: Anonymous WB Mouse 04/05/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse Liver Tissue
• Species: Mouse

Bioinformatics

• Gene Symbol: APOB
• Entrez:
  • 338(Human)
  • 238055(Mouse)