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Apolipoprotein B/ApoB Antibody

 (7 Publications)
 (2 Reviews)
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Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.
Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Apolipoprotein B/ApoB Antibody Summary

Immunogen
Mouse low density lipoprotein (LDL)
Localization
Secreted
Specificity
Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOB
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID:31807238).
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB200-527 in the following applications:
Publications
Read Publications using
NB200-527 in the following applications:

  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved. Rat reactivity reported in scientific literature (PMID: 29964320).

Packaging, Storage & Formulations

Storage
Store at -70C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Apolipoprotein B/ApoB Antibody

  • Apo B-100
  • APOB
  • ApoB-100
  • ApoB-48
  • apolipoprotein B (including Ag(x) antigen)
  • Apolipoprotein B
  • apolipoprotein B-100
  • apolipoprotein B48
  • FLDB
  • LDLCQ4
  • mutant Apo B 100

Background

Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ELISA: Lipoprotein a Antibody (4H1) [NBP2-37477] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western Blot: Lipoprotein a Antibody (4H1) [NBP2-37477] - Western blot analysis using LPA mAb against human LPA recombinant protein. (Expected MW is 34.1 kDa)
Immunocytochemistry/Immunofluorescence: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunofluorescence analysis of HepG2 cells using LPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Apolipoprotein
A-I/ApoA1 Antibody - ...
NBP2-52979
Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - An intracellular stain was performed on HepG2 cells with Apolipoprotein A-1/ApoA1 Antibody NBP2-52979 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .
Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Western blot analysis using Apolipoprotein A-I/ApoA1 antibody. Harvested from mouse, separated on a 4-12 gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 3% BSA/TBST. Probed with 1:8000 anti-apoA1 in 1%BSA/TBST, and detect edwith an anti-rabbit HRP secondary antibody using chemiluminescence. WB dilution 1: 8000, block before incubation overnight. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP2-52979] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Apolipoprotein A-1/ApoA1 Antibody (NBP2-52979) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
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Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. IHC-P image submitted by a verified customer review.
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Western Blot: MTTP Antibody [NBP1-62489] - HepG2 cell lysate.
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Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Lipase/LPL Antibody [ ...
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Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Western Blot: Metallothionein-1B Overexpression Lysate (Adult Normal) [NBP2-10437] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Metallothionein-1B.
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CD55/DAF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (left panel, positive staining) and MOLT-4 human acute lymphoblastic leukemia cell line (right panel, negative staining) using Goat Anti-Human CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2009) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Apolipoprotein
B/ApoB Antibody
NB200-527
Apolipoprotein B/ApoB Antibody
Species: Hu, Mu, Rt
Applications: WB, IP

Publications for Apolipoprotein B/ApoB Antibody (NB200-527)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IP, WB.


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Showing Publications 1 - 7 of 7.
Publications using NB200-527 Applications Species
Liu W, Sun C, Yan Y Et al. Hepatic P38 Activation Modulates Systemic Metabolism Through Fgf21-Mediated Interorgan Communication Diabetes Oct 21 2021 [PMID: 34675006]
Matsumoto A, Takahashi Y, Ogata K Et Al. Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in blood iScience Aug 1 2021 [PMID: 34368655]
Song Y, Kim S, Lee H et al. Chromenopyrimidinone Controls Stemness and Malignancy by suppressing CD133 Expression in Hepatocellular Carcinoma Cancers (Basel) May 8 2020 [PMID: 32397206] (WB, Human) WB Human
Matsumoto A, Takahashi Y, Chang H et al. Blood concentrations of small extracellular vesicles are determined by a balance between abundant secretion and rapid clearance J Extracell Vesicles Jan 1 2019 [PMID: 31807238] (IP, Mouse) IP Mouse
Howell GE, McDevitt E, Henein L et al. Alterations in cellular lipid metabolism produce neutral lipid accumulation following exposure to the organochlorine compound trans-nonachlor in rat primary hepatocytes. Environ. Toxicol. Jul 2 2018 [PMID: 29964320] (WB, Rat) WB Rat
Grefhorst A, van den Beukel JC, Dijk W et al. Multiple effects of cold exposure on livers of male mice J. Endocrinol. May 9 2018 [PMID: 29743343] (WB, Mouse) WB Mouse
Tsai WC, Hsu SD, Hsu CS et al. MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis J Clin Invest 2012 Aug 1 [PMID: 22820290] (WB, Mouse) WB Mouse

Reviews for Apolipoprotein B/ApoB Antibody (NB200-527) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB200-527:
Filter by Applications
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Images Ratings Applications Species Date Details
Western Blot Apolipoprotein B/ApoB NB200-527
Enlarge 		4
reviewed by:
Wenliang Zhang
 Western Blot Mouse 03/01/2018
View

Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesMouse
Lot2c08910
Western Blot Apolipoprotein B/ApoB NB200-527
Enlarge 		5
reviewed by:
Dina Schneider
 Western Blot Mouse 04/05/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse Liver Tissue
SpeciesMouse

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FAQs for Apolipoprotein B/ApoB Antibody (NB200-527) (0)

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Western Blot Apolipoprotein B/ApoB NB200-527
Enlarge
 Wenliang Zhang
03/01/2018
Application: Western Blot
Species: Mouse
read this review
Western Blot Apolipoprotein B/ApoB NB200-527
Enlarge
 Dina Schneider
04/05/2017
Application: Western Blot
Species: Mouse
read this review

Bioinformatics

Gene Symbol APOB
Entrez