Home » Apolipoprotein B/ApoB » Apolipoprotein B Antibodies » Apolipoprotein B/ApoB Antibody

Apolipoprotein B/ApoB Antibody

 (3 Publications)
 (2 Reviews)
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Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.
Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Apolipoprotein B/ApoB Antibody Summary

Immunogen
Mouse low density lipoprotein (LDL)
Localization
Secreted
Specificity
Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOB
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB200-527 in the following application:
Publications
Read Publications using
NB200-527 in the following applications:

  • WB
    3 publications

Reactivity Notes

Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved. Rat reactivity reported in scientific literature (PMID: 29964320).

Packaging, Storage & Formulations

Storage
Store at -70C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Apolipoprotein B/ApoB Antibody

  • Apo B-100
  • APOB
  • ApoB-100
  • ApoB-48
  • apolipoprotein B (including Ag(x) antigen)
  • Apolipoprotein B
  • apolipoprotein B-100
  • apolipoprotein B48
  • FLDB
  • LDLCQ4
  • mutant Apo B 100

Background

Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunofluorescence analysis of HepG2 cells using LPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Total protein from HepG2 and Hek293 cells was separated on a 4-15% gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-APOA1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
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Western Blot: MTTP Antibody [NBP1-62489] - HepG2 cell lysate, concentration 0.2-1 ug/ml.
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Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood leukocytes by mouse monoclonal anti-CD55 antibody MEM-118.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) PE-Cy(TM)5.
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Publications for Apolipoprotein B/ApoB Antibody (NB200-527)(3)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: WB.


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(3)
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Reviews for Apolipoprotein B/ApoB Antibody (NB200-527) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB200-527:
Filter by Applications
WB
(2)
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(2)
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Images Ratings Applications Species Date Details
Western Blot Apolipoprotein B/ApoB NB200-527
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reviewed by:
Anonymous
 WB Mouse 03/01/2018
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Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesMouse
Lot2c08910
Western Blot Apolipoprotein B/ApoB NB200-527
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reviewed by:
Anonymous
 WB Mouse 04/05/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse Liver Tissue
SpeciesMouse

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Western Blot Apolipoprotein B/ApoB NB200-527
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 Anonymous
03/01/2018
Application: WB
Species: Mouse
read this review
Western Blot Apolipoprotein B/ApoB NB200-527
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 Anonymous
04/05/2017
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol APOB
Entrez