Apolipoprotein A5 Antibody (1G5G9)

Images

 
Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis of ApoA5 in HepG2 lysates.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. ...read more
Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in ...read more
Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis using anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard.
Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Detection of Apo A5 in HepG2 cell lysate with NB400-139.

Product Details

Summary
Reactivity Hu, HaSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, ICC/IF (-)
Clone
1G5G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Apolipoprotein A5 Antibody (1G5G9) Summary

Immunogen
Ni-NTA purified full-length recombinant human Apolipoprotein A5 expressed in E. Coli strain BL21 (DE3). [UniProt# Q6Q788]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APOA5
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This Apolipoprotein A5 antibody is useful for ELISA, Western Blot and Immunohistochemistry paraffin embedded sections. In Western blot a band at approximately 41 kDa is seen. This antibody does not work in ICC/IF. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Apolipoprotein A5 Lysate (NBL1-07611)
Publications
Read Publication using NB400-139.

Reactivity Notes

Human. Immunogen sequence has 83% identity to bovine, 79% identity to rat and 78% identity to mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Apolipoprotein A5 Antibody (1G5G9)

  • APOAV
  • apo-AV
  • APOA-V
  • Apolipoprotein A5
  • apolipoprotein A-V
  • FLJ97995
  • MGC126836
  • RAP3MGC126838
  • Regeneration-associated protein 3

Background

Apolipoprotein A-V (APOA5) belongs to apolipoprotein A1/A4/E family and is a component of several lipoprotein fractions, including VLDL, HDL and chylomicrons. Expressed at high levels in plasma and liver, APOA5 exists as a secreted protein and interacts with GPIHBP1. APOA5 is an important determinant of plasma triglyceride levels by both behaving as a potent stimulator of apo-CII lipoprotein lipase/LPL TG hydrolysis and an inhibitor of hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). APOA5 activates lecithin: cholesterol acyltransferase (LCAT) but does not enhance efflux of cholesterol from macrophages. Transgenic mice overexpressing human APOA5 gene exhibit one-third lower plasma TG levels than wild type, whereas APOA5 knockout mice possess four times as much plasma TG levels, with no effect on plasma cholesterol levels. Clinically, defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR) and hyperlipoproteinemia type 5 (HLPP5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein A5 Antibody (NB400-139)(1)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APOA5
Entrez
Uniprot