Angiopoietin-2 Antibody

Western Blot: Angiopoietin-2 Antibody [NBP2-15386] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Angiopoietin 2 Antibody [NBP2-15386] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Angiopoietin 2 Antibody [NBP2-15386] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Western Blot: Angiopoietin-2 Antibody [NBP2-15386] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Angiopoietin-2 Antibody [NBP2-15386] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Product Discontinued

Angiopoietin-2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Angiopoietin 2. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Porcine (92%), Canine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANGPT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (85%), Bovine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Angiopoietin-2 Antibody

• AGPT2
• ANG2
• ANG-2
• angiopoietin 2
• Angiopoietin-2
• angiopoietin-2a
• angiopoietin-2B
• angiopoitin 2
• ANGPT2
• Tie2-ligand

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANGPT2
• Uniprot:
  • O15123()