Androgen R/NR3C4 Antibody (2H8)

Images

 
Western Blot: Androgen Receptor Antibody (2H8) [NBP1-47471] - Western blot analysis using Androgen Receptor mouse mAb against K562 (1), Jurkat (2) and LNCaP (3) cell lysates.
Immunohistochemistry-Paraffin: Androgen Receptor Antibody (2H8) [NBP1-47471] - Immunohistochemical analysis of paraffin-embedded human Prostate tissues using Androgen Receptor mouse mAb
Western Blot: Androgen Receptor Antibody (2H8) [NBP1-47471] - Western blot analysis using Androgen Receptor mouse mAb against Androgen Receptor (aa221-321)-hIgGFc transfected HEK293 cell lysate.
Western Blot: Androgen Receptor Antibody (2H8) [NBP1-47471] - Western blot analysis using Androgen Receptor mouse mAb against human recombinant Androgen Receptor.
Immunohistochemistry-Paraffin: Androgen Receptor Antibody (2H8) [NBP1-47471] - Immunohistochemical analysis of paraffin-embedded human testis (left) and prostate cancer tissues (right) using Androgen Receptor mouse mAb ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Androgen R/NR3C4 Antibody (2H8) Summary

Immunogen
Purified recombinant fragment of human Androgen Receptor expressed in E. coli. [UniProt# P10275]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AR
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This Androgen Receptor (2H8) antibody is useful in Western Blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
K-562 Lysate (NB820-59464)

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Androgen R/NR3C4 Antibody (2H8)

  • AIS
  • Androgen R
  • androgen receptor
  • AndrogenR
  • AR
  • DHTR
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • NR3C4
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SBMA
  • SMAX1
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the Androgen Receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain. The Androgen Receptor is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AR
Entrez
Uniprot