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Androgen R/NR3C4 Antibody (2H8)

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Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against K562 (1), Jurkat (2) and LNCaP (3) cell lysates.
Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against K562 (1), Jurkat (2) and LNCaP (3) cell lysates.
Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis of paraffin-embedded human Prostate tissues using Androgen Receptor mouse mAb
Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against Androgen Receptor (aa221-321)-hIgGFc transfected HEK293 cell lysate.
Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against human recombinant Androgen Receptor.
Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis of paraffin-embedded human testis (left) and prostate cancer tissues (right) using Androgen Receptor mouse mAb with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-47471AF350
Alexa Fluor 405
NBP1-47471AF405
Alexa Fluor 488
NBP1-47471AF488
Alexa Fluor 532
NBP1-47471AF532
Alexa Fluor 594
NBP1-47471AF594
Alexa Fluor 647
NBP1-47471AF647
Alexa Fluor 700
NBP1-47471AF700
Alexa Fluor 750
NBP1-47471AF750
Biotin
NBP1-47471B
DyLight 350
NBP1-47471UV
DyLight 405
NBP1-47471V
DyLight 488
NBP1-47471G
DyLight 550
NBP1-47471R
DyLight 594
NBP1-47471DL594
DyLight 650
NBP1-47471C
DyLight 680
NBP1-47471FR
DyLight 755
NBP1-47471IR
FITC
NBP1-47471F
HRP
NBP1-47471H
Janelia Fluor 549
NBP1-47471JF549
Janelia Fluor 646
NBP1-47471JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80576
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Androgen R/NR3C4 Antibody (2H8) Summary

Immunogen
Purified recombinant fragment of human Androgen Receptor expressed in E. coli. [UniProt# P10275]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AR
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This Androgen Receptor (2H8) antibody is useful in Western Blot, Immunohistochemistry on paraffin-embedded sections and ELISA.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
K-562 Whole Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Androgen R/NR3C4 Antibody (2H8)

  • AIS
  • Androgen R
  • androgen receptor
  • AndrogenR
  • AR
  • DHTR
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • NR3C4
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SBMA
  • SMAX1
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the Androgen Receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain. The Androgen Receptor is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Protein S/PROS1 Antibody [NBP1-87217] - Analysis using Anti-PROS1 antibody NBP1-87217 (A) shows similar pattern to independent antibody NBP1-87218 (B).
Immunohistochemistry-Paraffin: Protein S/PROS1 Antibody [NBP1-87217] - Staining of human testis.
Western Blot: Protein S/PROS1 Antibody [NBP1-87217] - Analysis in human plasma.
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Immunohistochemistry-Paraffin: PSAT1 Antibody [NBP1-32920] - NCI-N87 xenograft, using PSAT1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Control Lysate(s)

K-562 Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-47471AF350
Alexa Fluor 405 NBP1-47471AF405
Alexa Fluor 488 NBP1-47471AF488
Alexa Fluor 532 NBP1-47471AF532
Alexa Fluor 594 NBP1-47471AF594
Alexa Fluor 647 NBP1-47471AF647
Alexa Fluor 700 NBP1-47471AF700
Alexa Fluor 750 NBP1-47471AF750
Biotin NBP1-47471B
DyLight 350 NBP1-47471UV
DyLight 405 NBP1-47471V
DyLight 488 NBP1-47471G
DyLight 550 NBP1-47471R
DyLight 594 NBP1-47471DL594
DyLight 650 NBP1-47471C
DyLight 680 NBP1-47471FR
DyLight 755 NBP1-47471IR
FITC NBP1-47471F
HRP NBP1-47471H
Janelia Fluor 549 NBP1-47471JF549
Janelia Fluor 646 NBP1-47471JF646
Learn about Custom Antibody Labeling

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