Androgen R/NR3C4 Antibody (2H8) - Azide and BSA Free

Western Blot: Androgen R/NR3C4 Antibody (2H8) - Azide and BSA Free [NBP2-80576] - Analysis using Androgen Receptor mouse mAb against K562 (1), Jurkat (2) and LNCaP (3) cell lysates. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (2H8) - Azide and BSA Free [NBP2-80576] - Analysis of paraffin-embedded human Prostate tissues using Androgen Receptor mouse mAb Image from the standard format of this antibody.

Western Blot: Androgen R/NR3C4 Antibody (2H8) - Azide and BSA Free [NBP2-80576] - Analysis using Androgen Receptor mouse mAb against Androgen Receptor (aa221-321)-hIgGFc transfected HEK293 cell lysate. Image from the standard format of this antibody.

Western Blot: Androgen R/NR3C4 Antibody (2H8) - Azide and BSA Free [NBP2-80576] - Analysis using Androgen Receptor mouse mAb against human recombinant Androgen Receptor. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (2H8) - Azide and BSA Free [NBP2-80576] - Analysis of paraffin-embedded human testis (left) and prostate cancer tissues (right) using Androgen Receptor mouse mAb with DAB staining. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Androgen R/NR3C4 Antibody (2H8) - Azide and BSA Free Summary

• Immunogen: Purified recombinant fragment of human Androgen Receptor expressed in E. coli. [UniProt# P10275]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AR
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This Androgen Receptor (2H8) antibody is useful in Western Blot, Immunohistochemistry on paraffin-embedded sections and ELISA.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Androgen R/NR3C4 Antibody (2H8) - Azide and BSA Free

• AIS
• Androgen R
• androgen receptor
• AndrogenR
• AR
• DHTR
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• NR3C4
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SBMA
• SMAX1
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the Androgen Receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain. The Androgen Receptor is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AR