AMPK alpha 1 [p Ser496] Antibody

Images

 
Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Immunoblot analysis of total cell extracts from MIN6 cells grown in the medium containing indicated glucose using AMPK alpha 1 (Phospho-Ser496) Antibody. ...read more
Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Staining of methanol-fixed Hela cells using AMPK alpha 1(Phospho-Ser496) antibody
Immunohistochemistry-Paraffin: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Paraffin-embedded human colon carcinoma tissue using AMPK alpha 1 (Phospho-Ser496)Antibody (left) or the same antibody preincubated with ...read more
Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPK alpha1 (Phospho-Ser496) Antibody.
Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Analysis of extracts from HUVEC cells untreated or treated with UV using AMPK alpha 1(Phospho-Ser496)Antibody

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

AMPK alpha 1 [p Ser496] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human AMPK alpha1/AMPK2 around the phosphorylation site of serine 487 (S-G-SP-V-S).
Modification
p Ser496
Specificity
AMPK alpha 1/AMPK2 (specific to Phospho-Ser496) detects endogenous levels of AMPK alpha 1/AMPK2 only when phosphorylated at serine 496
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKAA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-81784 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AMPK alpha 1 [p Ser496] Antibody

  • AMP -activate kinase alpha 1 subunit
  • AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK alpha 1
  • AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
  • AMPK subunit alpha-1
  • AMPK
  • AMPK1
  • AMPKa1
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC33776
  • MGC57364
  • PRKAA1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostatis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AMPK alpha 1 Antibody (NB100-81784) (0)

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Review for AMPK alpha 1 Antibody (NB100-81784) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-81784:
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Images Ratings Applications Species Date Details
Western Blot AMPK alpha 1 NB100-81784
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reviewed by:
Kohsuke Kataoka
WB Mouse 02/07/2018
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Summary

ApplicationWestern Blot
Sample TestedMin6 mouse insulinoma whole cell lysate
SpeciesMouse
Lot5616

Product General Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

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Recent Reviews

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1
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Kohsuke Kataoka
02/07/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PRKAA1
Entrez
Uniprot