Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Immunoblot analysis of total cell extracts from MIN6 cells grown in the medium containing indicated glucose using AMPK alpha 1 (Phospho-Ser496) Antibody. ...read more
Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Staining of methanol-fixed Hela cells using AMPK alpha 1(Phospho-Ser496) antibody
Immunohistochemistry-Paraffin: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Paraffin-embedded human colon carcinoma tissue using AMPK alpha 1 (Phospho-Ser496)Antibody (left) or the same antibody preincubated with ...read more
Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPK alpha1 (Phospho-Ser496) Antibody.
Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Analysis of extracts from HUVEC cells untreated or treated with UV using AMPK alpha 1(Phospho-Ser496)Antibody
63 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NB100-81784 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for AMPK alpha 1 [p Ser496] Antibody
AMP -activate kinase alpha 1 subunit
AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
AMPK alpha 1
AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
AMPK subunit alpha-1
protein kinase, AMP-activated, alpha 1 catalytic subunit
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostatis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.