AMPK alpha 1 Antibody

Images

 
Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared ...read more
Immunohistochemistry: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by immunohistochemistry. Sample: FFPE section of human smooth muscle. Antibody: Affinity purified rabbit anti-AMPK alpha 1 ...read more
Immunoprecipitation: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm-OrSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

AMPK alpha 1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1
Predicted Species
Rat (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKAA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2 - 5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-239 in the following applications:

Publications
Read Publications using
NB100-239 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22767231).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AMPK alpha 1 Antibody

  • AMP -activate kinase alpha 1 subunit
  • AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK alpha 1
  • AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
  • AMPK subunit alpha-1
  • AMPK
  • AMPK1
  • AMPKa1
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC33776
  • MGC57364
  • PRKAA1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-239
Species: Hu, Mu, Rt, Pm-Or
Applications: WB, IHC, IHC-P, IP

Publications for AMPK alpha 1 Antibody (NB100-239)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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(2)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-239 Applications Species
Xie B, Zhao M, Song D et Al. Induction of autophagy and suppression of type I IFN secretion by CSFV Autophagy Mar 21 2020 [PMID: 32160078]
Guevara-Cruz M, Godinez-Salas Et, Sanchez-Tapia M et Al. Genistein stimulates insulin sensitivity through gut microbiota reshaping and skeletal muscle AMPK activation in obese subjects BMJ Open Diabetes Res Care Mar 1 2020 [PMID: 32152146] (WB, Human) WB Human
Foller M, Jaumann M, Dettling J et al. AMP-activated protein kinase in BK-channel regulation and protection against hearing loss following acoustic overstimulation FASEB J 2012 Oct [PMID: 22767231] (WB, Mouse) WB Mouse
Scheele C, Nielsen S, Kelly M et al. Satellite cells derived from obese humans with type 2 diabetes and differentiated into myocytes in vitro exhibit abnormal response to IL-6. PLoS One 2012 [PMID: 22761857]
Wang S, Dale GL, Song P et al. AMPKalpha1 deletion shortens erythrocyte life span in mice: role of oxidative stress. J Biol Chem 2010 Jun [PMID: 20392689]
Bendayan M, Londono I, Kemp BE et al. Association of AMP-activated protein kinase subunits with glycogen particles as revealed in situ by immunoelectron microscopy. J Histochem Cytochem 2009 Oct [PMID: 19581628]
Hou X, Xu S, Maitland-Toolan KA et al. SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase. J Biol Chem 2008 Jul [PMID: 18482975]
Gauthier MS, Miyoshi H, Souza SC et al. AMP-activated protein kinase is activated as a consequence of lipolysis in the adipocyte: potential mechanism and physiological relevance. J Biol Chem 2008 Jun [PMID: 18390901]
Qin S, Ni M, De Vries GW et al. Implication of S-adenosylhomocysteine hydrolase in inhibition of TNF-alpha- and IL-1beta-induced expression of inflammatory mediators by AICAR in RPE cells. Invest Ophthalmol Vis Sci 2008 Mar [PMID: 18326758]
Cammisotto PG, Londono I, Gingras D et al. Control of glycogen synthase through ADIPOR1-AMPK pathway in renal distal tubules of normal and diabetic rats. Am J Physiol Renal Physiol 2008 Apr [PMID: 18256313]
Show All 14 Publications.

Review for AMPK alpha 1 Antibody (NB100-239) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-239:
Filter by Applications
WB
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Rat 12/08/2011
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Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat

Blocking

Blocking DetailsBlocking Buffer: 5% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AMPK alpha 1 Antibody (NB100-239) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose NB600-396
Alexa Fluor 350 NB100-239AF350
Alexa Fluor 405 NB100-239AF405
Alexa Fluor 488 NB100-239AF488
Alexa Fluor 532 NB100-239AF532
Alexa Fluor 594 NB100-239AF594
Alexa Fluor 647 NB100-239AF647
Alexa Fluor 700 NB100-239AF700
Alexa Fluor 750 NB100-239AF750
Biotin NB100-239B
DyLight 350 NB100-239UV
DyLight 405 NB100-239V
DyLight 488 NB100-239G
DyLight 550 NB100-239R
DyLight 594 NB100-239DL594
DyLight 650 NB100-239C
DyLight 680 NB100-239FR
DyLight 755 NB100-239IR
FITC NB100-239F
HRP NB100-239H
Janelia Fluor 549 NB100-239JF549
Janelia Fluor 646 NB100-239JF646

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Blogs on AMPK alpha 1.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

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Recent Reviews

4
5
0
4
1
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2
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1
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Anonymous
12/08/2011
Application: WB
Species: Rat

Bioinformatics

Gene Symbol PRKAA1
Entrez
Uniprot