AMPK alpha 1 Antibody

Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by immunohistochemistry. Sample: FFPE section of human smooth muscle. Antibody: Affinity purified rabbit anti-AMPK alpha 1 (NB100-239). Detection: DAB

Immunoprecipitation: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 (lot 2) used for IP at 3 ug per reaction. AMPK alpha 1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated AMPK alpha 1, NB100-239 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu, Rt, Pm-Or
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

AMPK alpha 1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1
• Predicted Species: Rat (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22767231).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AMPK alpha 1 Antibody

• AMP -activate kinase alpha 1 subunit
• AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
• AMPK alpha 1
• AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
• AMPK subunit alpha-1
• AMPK
• AMPK1
• AMPKa1
• EC 2.7.11
• EC 2.7.11.1
• MGC33776
• MGC57364
• PRKAA1
• protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMPK alpha 1 Antibody (NB100-239)(15)

Publications using NB100-239

• Xie B, Zhao M, Song D et Al. Induction of autophagy and suppression of type I IFN secretion by CSFV Autophagy Mar 21 2020 [PMID: 32160078]
• Guevara-Cruz M, Godinez-Salas Et, Sanchez-Tapia M et Al. Genistein stimulates insulin sensitivity through gut microbiota reshaping and skeletal muscle AMPK activation in obese subjects BMJ Open Diabetes Res Care Mar 1 2020 [PMID: 32152146] (WB, Human)
• Harun-Or-Rashid, M, Pappenhagen, N Et al. Structural and Functional Rescue of Chronic Metabolically Stressed Optic Nerves through Respiration. J Neurosci May 30 2018 [PMID: 29760184] (WB, Mouse)
• Foller M, Jaumann M, Dettling J et al. AMP-activated protein kinase in BK-channel regulation and protection against hearing loss following acoustic overstimulation FASEB J 2012 Oct [PMID: 22767231] (WB, Mouse)
• Scheele C, Nielsen S, Kelly M et al. Satellite cells derived from obese humans with type 2 diabetes and differentiated into myocytes in vitro exhibit abnormal response to IL-6. PLoS One 2012 [PMID: 22761857]
• Wang S, Dale GL, Song P et al. AMPKalpha1 deletion shortens erythrocyte life span in mice: role of oxidative stress. J Biol Chem 2010 Jun [PMID: 20392689]
• Bendayan M, Londono I, Kemp BE et al. Association of AMP-activated protein kinase subunits with glycogen particles as revealed in situ by immunoelectron microscopy. J Histochem Cytochem 2009 Oct [PMID: 19581628]
• Hou X, Xu S, Maitland-Toolan KA et al. SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase. J Biol Chem 2008 Jul [PMID: 18482975]
• Gauthier MS, Miyoshi H, Souza SC et al. AMP-activated protein kinase is activated as a consequence of lipolysis in the adipocyte: potential mechanism and physiological relevance. J Biol Chem 2008 Jun [PMID: 18390901]
• Qin S, Ni M, De Vries GW et al. Implication of S-adenosylhomocysteine hydrolase in inhibition of TNF-alpha- and IL-1beta-induced expression of inflammatory mediators by AICAR in RPE cells. Invest Ophthalmol Vis Sci 2008 Mar [PMID: 18326758]
• Cammisotto PG, Londono I, Gingras D et al. Control of glycogen synthase through ADIPOR1-AMPK pathway in renal distal tubules of normal and diabetic rats. Am J Physiol Renal Physiol 2008 Apr [PMID: 18256313]
• Qin S, De Vries GW. alpha2 But not alpha1 AMP-activated protein kinase mediates oxidative stress-induced inhibition of retinal pigment epithelium cell phagocytosis of photoreceptor outer segments. J Biol Chem 2008 Mar [PMID: 18195011]
• Zang M, Xu S, Maitland-Toolan KA et al. Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclerosis in diabetic LDL receptor-deficient mice. Diabetes 2006 Aug [PMID: 16873680]
• Doi M, Yamaoka I, Nakayama M et al. Isoleucine, a blood glucose-lowering amino acid, increases glucose uptake in rat skeletal muscle in the absence of increases in AMP-activated protein kinase activity. J Nutr 2005 Sep [PMID: 16140883]
• Zang M, Zuccollo A, Hou X et al. AMP-activated protein kinase is required for the lipid-lowering effect of metformin in insulin-resistant human HepG2 cells. J Biol Chem 2004 Nov [PMID: 15371448]

Review for AMPK alpha 1 Antibody (NB100-239) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Chuen Ju WB Rat 12/08/2011

Summary

• Application: Western Blot
• Sample Tested: Rat
• Species: Rat

Bioinformatics

• Gene Symbol: PRKAA1
• Entrez:
  • 5562(Human)
  • 105787(Mouse)
  • 65248(Rat)
• Uniprot:
  • Q13131()