AMPK alpha 1 Antibody

Images

 
Western Blot: AMPK alpha 1 Antibody [NB100-239] - Extracts from 1. bovine aortic endothelial cells, 2. rat aortic smooth muscle cells, 3. HepG2 cells, or 4. human aortic endothelial cells. Antibody used at 2 ug/ml.
Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody [NB100-239] - Human muscle; Antibody used at a dilution of 1:5,000 (0.2ug/ml).

Product Details

Summary
Reactivity Hu, Rt, Bv, Mu, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

AMPK alpha 1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1
Predicted Species
Mouse (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKAA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 2-8 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval is not recommended.
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-239 in the following applications:

Publications
Read Publications using
NB100-239 in the following applications:

  • WB
    1 publication

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AMPK alpha 1 Antibody

  • AMP -activate kinase alpha 1 subunit
  • AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK alpha 1
  • AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
  • AMPK subunit alpha-1
  • AMPK
  • AMPK1
  • AMPKa1
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC33776
  • MGC57364
  • PRKAA1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AMPK alpha 1 Antibody (NB100-239)(12)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-239 Applications Species
Foller M, Jaumann M, Dettling J et al. AMP-activated protein kinase in BK-channel regulation and protection against hearing loss following acoustic overstimulation FASEB J 2012 Oct [PMID: 22767231] (WB, Mouse) WB Mouse
Scheele C, Nielsen S, Kelly M et al. Satellite cells derived from obese humans with type 2 diabetes and differentiated into myocytes in vitro exhibit abnormal response to IL-6. PLoS One 2012 [PMID: 22761857]
Wang S, Dale GL, Song P et al. AMPKalpha1 deletion shortens erythrocyte life span in mice: role of oxidative stress. J Biol Chem 2010 Jun [PMID: 20392689]
Bendayan M, Londono I, Kemp BE et al. Association of AMP-activated protein kinase subunits with glycogen particles as revealed in situ by immunoelectron microscopy. J Histochem Cytochem 2009 Oct [PMID: 19581628]
Hou X, Xu S, Maitland-Toolan KA et al. SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase. J Biol Chem 2008 Jul [PMID: 18482975]
Gauthier MS, Miyoshi H, Souza SC et al. AMP-activated protein kinase is activated as a consequence of lipolysis in the adipocyte: potential mechanism and physiological relevance. J Biol Chem 2008 Jun [PMID: 18390901]
Qin S, Ni M, De Vries GW et al. Implication of S-adenosylhomocysteine hydrolase in inhibition of TNF-alpha- and IL-1beta-induced expression of inflammatory mediators by AICAR in RPE cells. Invest Ophthalmol Vis Sci 2008 Mar [PMID: 18326758]
Cammisotto PG, Londono I, Gingras D et al. Control of glycogen synthase through ADIPOR1-AMPK pathway in renal distal tubules of normal and diabetic rats. Am J Physiol Renal Physiol 2008 Apr [PMID: 18256313]
Qin S, De Vries GW. alpha2 But not alpha1 AMP-activated protein kinase mediates oxidative stress-induced inhibition of retinal pigment epithelium cell phagocytosis of photoreceptor outer segments. J Biol Chem 2008 Mar [PMID: 18195011]
Zang M, Xu S, Maitland-Toolan KA et al. Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclerosis in diabetic LDL receptor-deficient mice. Diabetes 2006 Aug [PMID: 16873680]
Show All 12 Publications.

Review for AMPK alpha 1 Antibody (NB100-239) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-239:
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WB
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Rat 12/08/2011
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Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat

Blocking

Blocking DetailsBlocking Buffer: 5% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for AMPK alpha 1 Antibody (NB100-239) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for AMPK alpha 1 Antibody (NB100-239)

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Diseases for AMPK alpha 1 Antibody (NB100-239)

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Pathways for AMPK alpha 1 Antibody (NB100-239)

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PTMs for AMPK alpha 1 Antibody (NB100-239)

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Research Areas for AMPK alpha 1 Antibody (NB100-239)

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Blogs on AMPK alpha 1.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

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Recent Reviews

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Anonymous
12/08/2011
Application: WB
Species: Rat

Bioinformatics

Gene Symbol PRKAA1
Entrez
Uniprot