AMPK alpha 1/2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of U2OS cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded rat lung using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of NIH/3T3 cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of PC-12 cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded human colon using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded mouse kidney using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: AMPK alpha 1/2 Antibody [NBP3-05628] -Analysis of various lysates, using AMPKa1/AMPKa2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 45s.

Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [AMPK alpha 1/2] - Immunofluorescence analysis of NIH/3T3 cells using AMPK alpha 1/2 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [AMPK alpha 1/2] - Immunofluorescence analysis of MCF7 cells using AMPK alpha 1/2 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AMPK alpha 1/2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AMPK alpha 1/2 Antibody - BSA Free (NBP3-05628) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 13-300 of human PRKAA1/PRKAA2 (NP_006242.5). AEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMID
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for AMPK alpha 1/2 Antibody - BSA Free

• AMPK alpha 1/2
• AMPK
• AMPKa1
• PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAA1