alpha Tubulin 4a Antibody

Images

 
Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 10% SDS PAGE gel.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody [NBP2-21604] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin 4a protein stained by alpha Tubulin 4a antibody ...read more
Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - Paraffin-embedded rat brain. alpha Tubulin 4a antibody diluted at 1:500.
Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - A. 20 ug 293T whole cell lysate/extract B. 10 ug 293T whole cell lysate/extract C. 5 ug 293T whole cell lysate/extract 10% SDS-PAGE alpha Tubulin 4a antibody ...read more
Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - A. 30 ug Drosophila lysate/extract 10% SDS-PAGE alpha Tubulin 4a antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to ...read more
Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:10000. The ...read more
Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:20000. The ...read more
Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted at 1:20000. The ...read more
Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted at 1:20000. The ...read more
Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - Paraffin-embedded mouse brain. alpha Tubulin 4a antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Dr, Bv, Pm, Sh, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Order Details

alpha Tubulin 4a Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 4a. The exact sequence is proprietary.
Localization
Cytoplasm; Cytoskeleton; Microtubule
Predicted Species
Bovine (100%), Sheep (100%), Primate (99%), Xenopus (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBA4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%), Chimpanzee (99%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha Tubulin 4a Antibody

  • alpha 1 (testis specific)
  • Alpha-tubulin 1
  • FLJ30169
  • H2-ALPHA
  • Testis-specific alpha-tubulin
  • TUBA1
  • Tubulin alpha-1 chain
  • tubulin alpha-4A chain
  • Tubulin H2-alpha
  • tubulin, alpha 1
  • tubulin, alpha 4a

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin 4a Antibody (NBP2-21604) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for alpha Tubulin 4a Antibody (NBP2-21604)

Discover related pathways, diseases and genes to alpha Tubulin 4a Antibody (NBP2-21604). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on alpha Tubulin 4a.

Tubulin alpha 4A - A ubiquitous tubulin isoform linked to ALS and infertility
Microtubules are a main component of the cytoskeleton and play essential roles in a variety of cellular processes. These highly dynamic tubular structures are assembled from alpha- and beta-tubulin dimers to form a complex structural network of mic...  Read full blog post.

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Bioinformatics

Gene Symbol TUBA4A