alpha Tubulin 4a Antibody - BSA Free

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 10% SDS PAGE gel.

Western Blot: alpha Tubulin 4a Antibody [NBP2-21604] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - Rat esophagus. alpha Tubulin 4a stained by alpha Tubulin 4a antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody [NBP2-21604] - alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin 4a stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500.Blue: Fluoroshield with DAPI .

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis.A. 30 ug Drosophila lysate/extract10% SDS-PAGEalpha Tubulin antibody (NBP2-21604) dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin stained by alpha Tubulin antibody (NBP2-21604) diluted at 1:500.Blue: Fluoroshield with DAPI .

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (NBP2-21604) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (NBP2-21604) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (NBP2-21604) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ce, Ch, Dr, I, Pr, Bv, Pm, Sh, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

alpha Tubulin 4a Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit alpha Tubulin 4a Antibody - BSA Free (NBP2-21604) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 4a. The exact sequence is proprietary.
• Localization: Cytoplasm; Cytoskeleton; Microtubule
• Predicted Species: Bovine (100%), Chimpanzee (99%), Sheep (100%), Xenopus (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBA4A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mosquito (100%), Xenopus laevis (92%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for alpha Tubulin 4a Antibody - BSA Free

• alpha 1 (testis specific)
• alpha Tubulin 4a
• Alpha-tubulin 1
• FLJ30169
• H2-ALPHA
• Testis-specific alpha-tubulin
• TUBA1
• TUBA4A
• Tubulin alpha-1 chain
• tubulin alpha-4A chain
• Tubulin H2-alpha
• tubulin, alpha 1
• tubulin, alpha 4a

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBA4A