Western Blot: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-alpha-Tubulin Clone: B-5-1-2. The antibody was developed using Goat ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Staining of HEK293T cells transfected with a recombinant protein HA tagged, detected with Anti-HA Mouse primary antibody and ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal ...read more
ICC: Concentration is recommended using cultured chicken fibroblasts (CFB). WB: Concentration is recommended using a total cell extract of the human foreskin fibroblast cell line (FS11). Predicted molecular weight: 50 kDa.
Cross-reacts with African Green Monkey, Chlamydomonas reinhardtii, kangaroo rat and Sea Urchin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM PBS (pH 7.4)
15mM Sodium Azide
Protein A or G purified
Alternate Names for alpha Tubulin 4a Antibody (B-5-1-2)
alpha 1 (testis specific)
Tubulin alpha-1 chain
tubulin alpha-4A chain
tubulin, alpha 1
tubulin, alpha 4a
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Nicholas C. Mouse Polyomavirus T Antigens: Directors of Cell Cycle Signaling. Thesis. 2015 (WB, Mouse)
Details: alpha Tubulin 4a antibody clone B-5-1-2 was used for WB analysis of lysates from C57 MEFs that were infected or not with MPyVs RA, NG59, and 808A (withan UI mock-infected control) for 1.5 hours. Tubulin immunoblots were used for normalization purpose for blots of NBS1, MRE11, RAD50, LTag / Large Tumor Antigen and LTAg (Figure 7).
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