Novus Biologicals products are now on bio-techne.com

alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free

Images

 
Western Blot: alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-alpha-Tubulin Clone: B-5-1-2. The antibody was developed using ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Staining of HEK293T cells transfected with a recombinant protein HA tagged, detected with Anti-HA Mouse primary antibody and ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal ...read more
alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304] - Chicken fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 ug/mL Anti-alpha-Tubulin antibody, ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IP, MA
Clone
B-5-1-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
2.0 mg/ml

Order Details

alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free Summary

Immunogen
Sarkosyl-resistant filaments from Stronglycentrotus purpuratus (sea urchin).
Localization
Cytoplasmic
Specificity
Recognizes an epitope located at the C-terminal end of the alpha-tubulin isoform in a variety of organisms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBA4A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.5-1 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Simple Western
  • Western Blot 0.25-0.5 ug/ml
Application Notes
ICC: Concentration is recommended using cultured chicken fibroblasts (CFB). WB: Concentration is recommended using a total cell extract of the human foreskin fibroblast cell line (FS11). Predicted molecular weight: 50 kDa.
Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50; matrix was 12-230 kDa.
Theoretical MW
~50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Tubulin alpha-1B Overexpression Lysate
Publications
Read Publications using
NB120-11304 in the following applications:

  • WB
    3 publications

Reactivity Notes

Cross-reacts with African Green Monkey, Chlamydomonas reinhardtii, kangaroo rat and Sea Urchin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
2.0 mg/ml
Purity
Protein A or G purified

Alternate Names for alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free

  • alpha 1 (testis specific)
  • alpha Tubulin 4a
  • Alpha-tubulin 1
  • FLJ30169
  • H2-ALPHA
  • Testis-specific alpha-tubulin
  • TUBA1
  • TUBA4A
  • Tubulin alpha-1 chain
  • tubulin alpha-4A chain
  • Tubulin H2-alpha
  • tubulin, alpha 1
  • tubulin, alpha 4a

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1):53-60) in cells undergoing adipose differentiation actin synthesis decreases by 90%. Excellent as a protein loading control antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-221
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
NB500-317
Species: Bv(-), Ca(-), Ch(-), Hu, Mu(-), Rb(-)
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA, WB
NBP1-33527
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-93302
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
H00006908-Q01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
NB600-533
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IP, Simple Western, WB
NBP2-19784
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, PLA, WB
U-100H
Species: Hu
Applications: EnzAct
NBP3-25485
Species: Ca, Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
DBD00
Species: Hu
Applications: ELISA
AF2065
Species: Mu
Applications: ICC, IHC, Simple Western, WB
NBP1-89105
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-690
Species: Av, Bv, Ca, Ch, ChHa, Dr, Fu, Gt, Gp, Ha, Hu, Pm, Mu, Pa, Po, Pm, Rb, Rt, Xp, Ye
Applications: CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, Simple Western, WB
NBP1-30993
Species: Ha, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-32972
Species: Ch, Pm, Hu, Pm, Mu, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-1992
Species: Bv, Ca, Ch, Dr, Gt, Gp, Ha, Hu, Pm, Mu, Po, Rb, Rt, Sh, Sq, Xp
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
H00009910-M05
Species: Hu
Applications: ELISA, IHC, IHC-P, WB
NBP1-82558
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB120-11304
Species: Hu, Mu, Rt, Bv, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IP, MA

Publications for alpha Tubulin 4a Antibody (NB120-11304)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
All Species
Showing Publications 1 - 4 of 4.
Publications using NB120-11304 Applications Species
Merritt EK, Stec MJ, Thalacker-Mercer A et al. Heightened muscle inflammation susceptibility may impair regenerative capacity in aging humans. J Appl Physiol (1985) 2013-09-01 [PMID: 23681911] (Human) Human
Nicholas C. Mouse Polyomavirus T Antigens: Directors of Cell Cycle Signaling. Thesis. 2015-01-01 (WB, Mouse)

Details:
alpha Tubulin 4a antibody clone B-5-1-2 was used for WB analysis of lysates from C57 MEFs that were infected or not with MPyVs RA, NG59, and 808A (withan UI mock-infected control) for 1.5 hours. Tubulin immunoblots were used for normalization purpose for blots of NBS1, MRE11, RAD50, LTag / Large Tumor Antigen and LTAg (Figure 7).
WB Mouse
Ochaba J, Monteys AM, O'Rourke JG et al. PIAS1 Regulates Mutant Huntingtin Accumulation and Huntington's Disease-Associated Phenotypes In Vivo. Neuron. 2016-05-04 [PMID: 27146268] (WB, Mouse) WB Mouse
Zaki MS, Bhat G, Sultan T et al. PYCR2 mutations cause a lethal syndrome of microcephaly and failure to thrive. Ann. Neurol. 2016-04-30 [PMID: 27130255] (WB, Human) WB Human

Reviews for alpha Tubulin 4a Antibody (NB120-11304) (0)

There are no reviews for alpha Tubulin 4a Antibody (NB120-11304). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin 4a Antibody (NB120-11304) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional alpha Tubulin 4a Products

Research Areas for alpha Tubulin 4a Antibody (NB120-11304)

Find related products by research area.

Blogs on alpha Tubulin 4a.

Tubulin alpha 4A - A ubiquitous tubulin isoform linked to ALS and infertility
Microtubules are a main component of the cytoskeleton and play essential roles in a variety of cellular processes. These highly dynamic tubular structures are assembled from alpha- and beta-tubulin dimers to form a complex structural network of mic...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol TUBA4A
Entrez