Western Blot: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-alpha-Tubulin Clone: B-5-1-2. The antibody was developed using Goat ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Staining of HEK293T cells transfected with a recombinant protein HA tagged, detected with Anti-HA Mouse primary antibody and ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal ...read more
ICC: Concentration is recommended using cultured chicken fibroblasts (CFB). WB: Concentration is recommended using a total cell extract of the human foreskin fibroblast cell line (FS11). Predicted molecular weight: 50 kDa. Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50; matrix was 12-230 kDa.
Cross-reacts with African Green Monkey, Chlamydomonas reinhardtii, kangaroo rat and Sea Urchin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Nicholas C. Mouse Polyomavirus T Antigens: Directors of Cell Cycle Signaling. Thesis. 2015 (WB, Mouse)
Details: alpha Tubulin 4a antibody clone B-5-1-2 was used for WB analysis of lysates from C57 MEFs that were infected or not with MPyVs RA, NG59, and 808A (withan UI mock-infected control) for 1.5 hours. Tubulin immunoblots were used for normalization purpose for blots of NBS1, MRE11, RAD50, LTag / Large Tumor Antigen and LTAg (Figure 7).
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