alpha Tubulin 4a Antibody (B-5-1-2)

Images

 
Western Blot: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-alpha-Tubulin Clone: B-5-1-2. The antibody was developed using Goat ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Staining of HEK293T cells transfected with a recombinant protein HA tagged, detected with Anti-HA Mouse primary antibody and ...read more
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, PmSpecies Glossary
Applications WB, ICC/IF, IP, MiAr
Clone
B-5-1-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.3 mg/ml

alpha Tubulin 4a Antibody (B-5-1-2) Summary

Immunogen
Sarkosyl-resistant filaments from Stronglycentrotus purpuratus (sea urchin).
Localization
Cytoplasmic
Specificity
Recognizes an epitope located at the C-terminal end of the alpha-tubulin isoform in a variety of organisms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBA4A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Microarray
Application Notes
ICC: Concentration is recommended using cultured chicken fibroblasts (CFB). WB: Concentration is recommended using a total cell extract of the human foreskin fibroblast cell line (FS11). Predicted molecular weight: 50 kDa.
Control
Tubulin alpha-1B Overexpression Lysate
alpha Tubulin 4a Knockout HeLa Cell Lysate
Publications
Read Publications using
NB120-11304 in the following applications:

  • WB
    3 publications

Reactivity Notes

Cross-reacts with African Green Monkey, Chlamydomonas reinhardtii, kangaroo rat and Sea Urchin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
2.3 mg/ml
Purity
Protein A or G purified

Alternate Names for alpha Tubulin 4a Antibody (B-5-1-2)

  • alpha 1 (testis specific)
  • Alpha-tubulin 1
  • FLJ30169
  • H2-ALPHA
  • Testis-specific alpha-tubulin
  • TUBA1
  • Tubulin alpha-1 chain
  • tubulin alpha-4A chain
  • Tubulin H2-alpha
  • tubulin, alpha 1
  • tubulin, alpha 4a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha Tubulin 4a Antibody (NB120-11304)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
All Species
Showing Publications 1 - 4 of 4.
Publications using NB120-11304 Applications Species
Merritt EK, Stec MJ, Thalacker-Mercer A et al. Heightened muscle inflammation susceptibility may impair regenerative capacity in aging humans. J Appl Physiol (1985) 2013 Sep [PMID: 23681911] (Human) Human
Nicholas C. Mouse Polyomavirus T Antigens: Directors of Cell Cycle Signaling. Thesis. 2015 (WB, Mouse)

Details:
alpha Tubulin 4a antibody clone B-5-1-2 was used for WB analysis of lysates from C57 MEFs that were infected or not with MPyVs RA, NG59, and 808A (withan UI mock-infected control) for 1.5 hours. Tubulin immunoblots were used for normalization purpose for blots of NBS1, MRE11, RAD50, LTag / Large Tumor Antigen and LTAg (Figure 7).
WB Mouse
Ochaba J, Monteys AM, O'Rourke JG et al. PIAS1 Regulates Mutant Huntingtin Accumulation and Huntington's Disease-Associated Phenotypes In Vivo. Neuron. 2016 May 04 [PMID: 27146268] (WB, Mouse) WB Mouse
Zaki MS, Bhat G, Sultan T et al. PYCR2 mutations cause a lethal syndrome of microcephaly and failure to thrive. Ann. Neurol. Apr 30 2016 [PMID: 27130255] (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin 4a Antibody (NB120-11304) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-11304AF350
Alexa Fluor 405 NB120-11304AF405
Alexa Fluor 488 NB120-11304AF488
Alexa Fluor 532 NB120-11304AF532
Alexa Fluor 594 NB120-11304AF594
Alexa Fluor 647 NB120-11304AF647
Alexa Fluor 700 NB120-11304AF700
Alexa Fluor 750 NB120-11304AF750
DyLight 350 NB120-11304UV
DyLight 405 NB120-11304V
DyLight 488 NB120-11304G
DyLight 550 NB120-11304R
DyLight 594 NB120-11304DL594
DyLight 680 NB120-11304FR
DyLight 755 NB120-11304IR
Janelia Fluor 549 NB120-11304JF549
Janelia Fluor 646 NB120-11304JF646

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Blogs on alpha Tubulin 4a.

Tubulin alpha 4A - A ubiquitous tubulin isoform linked to ALS and infertility
Microtubules are a main component of the cytoskeleton and play essential roles in a variety of cellular processes. These highly dynamic tubular structures are assembled from alpha- and beta-tubulin dimers to form a complex structural network of mic...  Read full blog post.

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Bioinformatics

Gene Symbol TUBA4A
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