alpha-Smooth Muscle Actin Antibody (4A4)

Images

 
Western Blot: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis using ACTA2 mouse mAb against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell ...read more
Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of HepG2 cells using ACTA2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 ...read more
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of human duodenum tissues (left) and human esophagus tissues (right) using ACTA2 mouse mAb with DAB staining.
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple).
ELISA: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
4A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

alpha-Smooth Muscle Actin Antibody (4A4) Summary

Immunogen
Synthesized peptide of human Actin Alpha 2 Smooth Muscle. [UniProt# P62736]
Marker
Mesenchymal Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTA2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
This Actin Alpha 2 Smooth Muscle (4A4) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for alpha-Smooth Muscle Actin Antibody (4A4)

  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • ACTSA
  • ACTVS
  • alpha 2 actin
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • alpha-Smooth Muscle Actin
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • SMA
  • smooth muscle actin

Background

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Smooth Muscle Actin Antibody (NBP2-22120) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for alpha-Smooth Muscle Actin Antibody (NBP2-22120)

Discover related pathways, diseases and genes to alpha-Smooth Muscle Actin Antibody (NBP2-22120). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on alpha-Smooth Muscle Actin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

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Bioinformatics

Gene Symbol ACTA2
Entrez
Uniprot