alpha-Smooth Muscle Actin Antibody (1H8)

Images

 
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple).
Western Blot: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 ...read more
Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
ELISA: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
1H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

alpha-Smooth Muscle Actin Antibody (1H8) Summary

Immunogen
Purified recombinant fragment of human ACTA2 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTA2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-37641.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for alpha-Smooth Muscle Actin Antibody (1H8)

  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • ACTSA
  • ACTVS
  • alpha 2 actin
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • alpha-Smooth Muscle Actin
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • SMA
  • smooth muscle actin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Smooth Muscle Actin Antibody (NBP2-37641)(1)

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for alpha-Smooth Muscle Actin Antibody (NBP2-37641)

Discover related pathways, diseases and genes to alpha-Smooth Muscle Actin Antibody (NBP2-37641). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for alpha-Smooth Muscle Actin Antibody (NBP2-37641)

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Blogs on alpha-Smooth Muscle Actin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

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Bioinformatics

Gene Symbol ACTA2