alpha-Smooth Muscle Actin Antibody (1A4/asm-1)

Images

 
Western Blot: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. ...read more
Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Feline tissue stained with alpha-Smooth Muscle Actin Antibody at dilution of 1:600. Vascular wall was stained. IHC image ...read more
Simple Western: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Simple Western lane view shows a specific band for an observed molecular weight at ~45 kDa for alpha-Smooth Muscle Actin in 0.2 mg/ml of h. ...read more
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - An intracellular stain was performed on HeLa cells with NBP2-34522AF700 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Canine brain/spinal cord section. Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) staining in red. Endothelial cell marker in ...read more
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - M2 macrophage-related protein expression and the co-location of M2-macrophages and GC-MSCs within tumor tissues. ...read more
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522APC (blue) and a matched ...read more
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Staining of human Leiomyosarcoma statined with the Azide and BSA-free version of alpha-Smooth Muscle Actin Antibody ...read more
Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522AF647 (blue) and a ...read more
Simple Western: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Simple Western lane view shows a specific band for an observed molecular weight for Smooth Muscle Actin in 0.2 mg/ml of h. Uterus lysate. ...read more
Simple Western: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Electropherogram image of the corresponding Simple Western lane. alpha-Smooth Muscle Actin antibody was used at 10 ug/ml dilution of h. Aorta ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Gp, Gt, Pm, Pm, Rb, ShSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
1A4/asm-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

alpha-Smooth Muscle Actin Antibody (1A4/asm-1) Summary

Immunogen
Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) is made to the N-Terminal decapeptide of Alpha smooth muscle isoform of actin and conjugated to KLH.
Localization
Cytoplasmic
Marker
Leiomyosarcoma Marker
Specificity
Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACTA2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Simple Western 10 ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:600
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • Flow (Intracellular)
Application Notes
ELISA (For coating, order NBP2-34522 (BSA free version of NBP2-33006)). IHC-F reported in a verified customer review.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP2-33006 in the following applications:

Publications
Read Publications using
NBP2-33006 in the following applications:

Reactivity Notes

Feline reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for alpha-Smooth Muscle Actin Antibody (1A4/asm-1)

  • 1a4 sma
  • 1a4 smooth muscle actin
  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • ACTSA
  • ACTVS
  • alpha 2 actin
  • alpha sma 1a4
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • alpha-Smooth Muscle Actin
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • sma 1a4
  • SMA
  • smooth muscle actin

Background

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2.El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G.,... Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M.,... Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Smooth Muscle Actin Antibody (NBP2-33006)(18)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: FLOW, Flow, ICC/IF, IHC, IHC-P, WB.


Filter By Application
FLOW
(1)
Flow
(3)
ICC/IF
(6)
IHC
(3)
IHC-P
(2)
WB
(2)
All Applications
Filter By Species
Human
(6)
Mouse
(7)
Rat
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NBP2-33006 Applications Species
Yang L, Han B, Zhang M et Al. Activation of BK Channels Prevents Hepatic Stellate Cell Activation and Liver Fibrosis Through the Suppression of TGF beta 1/SMAD3 and JAK/STAT3 Profibrotic Signaling Pathways Front Pharmacol Mar 6 2020 [PMID: 32210801] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Li S, Lin Q, Shao X, et al. Gastric cancer-derived mesenchymal stromal cells trigger M2 macrophage polarization that promotes metastasis and EMT in gastric cancer Cell Death Dis Dec 4 2019 [PMID: 31801938] (ICC/IF, Mouse) ICC/IF Mouse
GrUneboom A, Hawwari I, Weidner D et al. A network of trans-cortical capillaries as mainstay for blood circulation in long bones Nat Metab. 2019 Feb [PMID: 31620676] (ICC/IF, Human)

Details:
Citation used the Alexa Fluor 647 format of this antibody.
ICC/IF Human
Kangawa A, Nishimura T, Nishimura T et al. Spontaneous Age-Related Histopathological Changes in Microminipigs Toxicol Pathol Jul 23 2019 [PMID: 31337280]
Chen YC, Gonzalez ME, Burman B et al. Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer Cell Rep Jun 25 2019 [PMID: 31242423] (IHC-P, Mouse) IHC-P Mouse
Karamched SR Targeted EDTA Chelation Therapy with Albumin Nanoparticles to Reverse Arterial Calcification and Restore Vascular Health in Chronic Kidney Disease Thesis (IHC, WB, Porcine) IHC, WB Porcine
Hreha TN, Collins CA, Daugherty AL et al. TGF beta 1 orchestrates renal fibrosis following Escherichia coli pyelonephritis Physiol Rep Mar 1 2020 [PMID: 32227630] (Flow, IHC-P, Mouse) Flow, IHC-P Mouse
Ruffenach G, Umar S, Vaillancourt M et al. Histological hallmarks and role of Slug/PIP axis in pulmonary hypertension secondary to pulmonary fibrosis EMBO Mol Med. 2019 Sep [PMID: 31468711] (ICC/IF, Human, Rat)

Details:
Citation used the Alexa Fluor 647 format of this antibody.
ICC/IF Human, Rat
Tan T, Hu H, Wang H et al Bioinspired lipoproteins-mediated photothermia remodels tumor stroma to improve cancer cell accessibility of second nanoparticles. Nat Commun. 2019 Jul 25 [PMID: 31346166] (FLOW, Mouse)

Details:
Citation using the Alexa Fluor 488 version of this antibody.
FLOW Mouse
An, S;Raju, I;Surenkhuu, B;Kwon, JE;Gulati, S;Karaman, M;Pradeep, A;Sinha, S;Mun, C;Jain, S; Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies Ocul Surf Apr 6 2019 [PMID: 30965123] (WB, Human) WB Human
Show All 18 Publications.

Reviews for alpha-Smooth Muscle Actin Antibody (NBP2-33006) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Canine, Feline.

Reviews using NBP2-33006:
Filter by Applications
IHC-Fr
(2)
All Applications
Filter by Species
Canine
(1)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen alpha-Smooth Muscle Actin NBP2-33006
Enlarge
reviewed by:
Anonymous
IHC-Fr Canine 12/16/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested brain and spinal cord
SpeciesCanine
LotIBR051902A

Comments

CommentsAlexa Fluor conjugated secondary antibodies were used
Immunohistochemistry-Frozen alpha-Smooth Muscle Actin NBP2-33006
Enlarge
reviewed by:
Anonymous
IHC-Fr Feline 02/07/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedVascular Smooth Muscle Cells
SpeciesFeline

Comments

CommentsAntibody dilution was 1:600

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Smooth Muscle Actin Antibody (NBP2-33006). (Showing 1 - 2 of 2 FAQs).

  1. I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
    • We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.
  2. Which antibodies would you recommend for the best performance in IHC?
    • NB300-978, NBP1-30894, NBP2-22120, NBP2-32808, NBP2-33006, and NBP2-34522 have the broadest IHC applications, as they additionally list IHC-Paraffin and IHC-Frozen. To narrow the list down further, we would recommend using either NB300-978 or NBP2-33006 since these products have the most ratings and cited publications for use in IHC methods. NB300-978 has IHC-Free-Floating listed as an application as well.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33006AF350
Alexa Fluor 405 NBP2-34522AF405
Alexa Fluor 488 NBP2-34522AF488
Alexa Fluor 532 NBP2-33006AF532
Alexa Fluor 594 NBP2-33006AF594
Alexa Fluor 647 NBP2-34522AF647
Alexa Fluor 700 NBP2-34522AF700
Alexa Fluor 750 NBP2-33006AF750
Allophycocyanin NBP2-34522APC
Biotin NBP2-34522B
DyLight 350 NBP2-34522UV
DyLight 405 NBP2-34522V
DyLight 488 NBP2-34522G
DyLight 550 NBP2-34522R
DyLight 594 NBP2-33006DL594
DyLight 650 NBP2-34522C
DyLight 680 NBP2-34522FR
DyLight 755 NBP2-34522IR
FITC NBP2-34522F
HRP NBP2-34522H
Janelia Fluor 549 NBP2-34522JF549
Janelia Fluor 646 NBP2-34522JF646
PE NBP2-34522PE
PerCP NBP2-34522PCP

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Blogs on alpha-Smooth Muscle Actin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
12/16/2019
Application: IHC-Fr
Species: Canine

Anonymous
02/07/2017
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol ACTA2
Uniprot