Western Blot: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. ...read more
Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Feline tissue stained with alpha-Smooth Muscle Actin Antibody at dilution of 1:600. Vascular wall was stained. IHC image ...read more
Simple Western: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Simple Western lane view shows a specific band for an observed molecular weight at ~45 kDa for alpha-Smooth Muscle Actin in 0.2 mg/ml of h. ...read more
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - An intracellular stain was performed on HeLa cells with NBP2-34522AF700 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Canine brain/spinal cord section. Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) staining in red. Endothelial cell marker in ...read more
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - M2 macrophage-related protein expression and the co-location of M2-macrophages and GC-MSCs within tumor tissues. ...read more
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522APC (blue) and a matched ...read more
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Staining of human Leiomyosarcoma statined with the Azide and BSA-free version of alpha-Smooth Muscle Actin Antibody ...read more
Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522AF647 (blue) and a ...read more
Simple Western: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Simple Western lane view shows a specific band for an observed molecular weight for Smooth Muscle Actin in 0.2 mg/ml of h. Uterus lysate. ...read more
Simple Western: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Electropherogram image of the corresponding Simple Western lane. alpha-Smooth Muscle Actin antibody was used at 10 ug/ml dilution of h. Aorta ...read more
Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) is made to the N-Terminal decapeptide of Alpha smooth muscle isoform of actin and conjugated to KLH.
Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Protein A purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
ELISA (For coating, order NBP2-34522 (BSA free version of NBP2-33006)). IHC-F reported in a verified customer review.
42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 5 using NBP2-33006 in the following applications:
Feline reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Store at -20C.
0.02% Sodium Azide
Protein A purified
Alternate Names for alpha-Smooth Muscle Actin Antibody (1A4/asm-1)
1a4 smooth muscle actin
Actin alpha 2
actin, alpha 2, smooth muscle, aorta
actin, aortic smooth muscle
alpha 2 actin
alpha sma 1a4
alpha smooth muscle actin
alphaSmooth Muscle Actin
alpha-Smooth Muscle Actin
Cell growth-inhibiting gene 46 protein
growth-inhibiting gene 46
smooth muscle actin
Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.
ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).
1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449
2.El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G.,... Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004
3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M.,... Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for alpha-Smooth Muscle Actin Antibody (NBP2-33006). (Showing 1 - 2 of 2 FAQs).
I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.
Which antibodies would you recommend for the best performance in IHC?
NB300-978, NBP1-30894, NBP2-22120, NBP2-32808, NBP2-33006, and NBP2-34522 have the broadest IHC applications, as they additionally list IHC-Paraffin and IHC-Frozen. To narrow the list down further, we would recommend using either NB300-978 or NBP2-33006 since these products have the most ratings and cited publications for use in IHC methods. NB300-978 has IHC-Free-Floating listed as an application as well.
Understanding Actin Alpha 2 Smooth Muscle Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated... Read full blog post.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.