alpha-Internexin Antibody (1D2) - BSA Free

Western Blot: alpha-Internexin Antibody (1D2) [NB300-216] - Analysis of different tissue lysates using alpha-Internexin antibody, dilution 1:10,000 (Green): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord. The alpha-Internexin antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa with slight variability among species.

Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody (1D2) [NB300-216] - Mixed cultures of rat CNS cells stained with NB300-216 (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.

Immunohistochemistry Free-Floating: alpha-Internexin Antibody (1D2) [NB300-216] - Analysis of rat cerebellum section stained with alpha-internexin antibody, dilution 1:5,000 (Green), and costained with chicken Calretinin pAb, dilution 1:2,000 (Red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The alpha-internexin antibody selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. Calretinin antibody stains interneurons predominantly in the molecular layer of the cerebellum.

• Reactivity: Hu, Mu, Rt, Fe
• Applications: WB, ICC/IF, IHC, IHC
• Clone: 1D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

alpha-Internexin Antibody (1D2) - BSA Free Summary

• Description: Novus Biologicals Mouse alpha-Internexin Antibody (1D2) - BSA Free (NB300-216) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-alpha-Internexin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant rat alpha-internexin expressed in and purified from E. coli.
• Epitope: The epitope for this antibody is not in the N-terminus; however exactly where it is located is not known. It is thought to be somewhere within the central alpha helical "rod" region of the molecule.
• Marker: Immature Neuronal Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: INA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:5000
  • Immunohistochemistry Free-Floating 1:5000
  • Immunohistochemistry 1:5000
  • Western Blot 1:10000
• Application Notes: This alpha Internexin antibody is useful for Immunohistochemistry-Paraffin and Western blot. A 64-66 kDa band can be seen in Western blotting.
• Theoretical MW: 64-66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and all mammalian species tested to date.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for alpha-Internexin Antibody (1D2) - BSA Free

• 66 kDa neurofilament protein
• alphaInternexin
• alpha-Internexin
• alpha-Inx
• FLJ18662
• FLJ57501
• INA
• internexin neuronal intermediate filament protein, alpha
• MGC12702
• NEF5
• neurofilament 5 (66kD)
• Neurofilament 5
• Neurofilament-66
• neurofilament-66, tax-binding protein
• NF-66
• NF-66alpha-internexin
• TXBP-1

Background

In mammalian CNS, five key neuronal intermediate filament (NIF) proteins have been discovered which includes alpha-internexin (66 kD, INA or Inexa), peripherin (57 kD), and three neurofilament (NF) proteins, which are neurofilament light (NF-L, 68 kD), medium (NF-M, 145 kD), and heavy (NF-H, 200 kD). Among NIFs, alpha-internexin is categorized as class IV NIF which is highly expressed in most neurons during development especially when they begin to differentiate and before the expression of NF triplet proteins, and it is structurally/functionally associated with NF triplet proteins inside mature CNS neurons. Peripherin is mainly expressed in PNS and in some CNS neurons also, and alpha-internexin - peripherin self-assemble or co-assemble with neurofilament protein subunits to form filamentous structure before their translocation into the axons to become an essential constituent of shape-maintaining IF network in mature neurons. It has been recognized as one of the many proteins phosphorylated by ATM/ATR in response to DNA damage and has been associated with developmental, degenerative /overexpression causes neuronal cell death, and inflammatory processes of CNS. Abnormal NIF accumulation is a neuropathological signature of several neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, dementia with Lewy bodies, and amyotrophic lateral sclerosis. Alpha-internexin has been detected as a major component of pathological inclusions in frontotemporal dementia, also called 'neuronal intermediate filament inclusion disease (NIFID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Internexin Antibody (NB300-216)(1)

Publication using NB300-216

• Yao F, Sumners C, O'Rourke ST et al. Angiotensin II increases GABAB receptor expression in nucleus tractus solitarii of rats. Am J Physiol Heart Circ Physiol; 294(6):H2712-2720. 2008-01-01 [PMID: 18424635] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: INA
• Entrez:
  • 9118(Human)