alpha 2-Macroglobulin Antibody

Western blot shows samples of purified a2-Macroglobulin from mouse sera. PVDF membrane was probed with 0.1 µg/mL Sheep Anti-Mouse a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5798) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for a2-Macroglobulin was detected at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha 2-Macroglobulin Antibody Summary

• Immunogen: Mouse serum-derived alpha ₂-Macroglobulin
• Specificity: Detects mouse alpha ₂-Macroglobulin in direct ELISAs and Western blots. In direct ELISA, less than 2% cross-reactivity with human alpha ₂‑Macroglobulin, recombinant rat (rr) alpha ₂-Macroglobulin, and rr beta ₂-Macroglobulin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: A2M
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 2-Macroglobulin Antibody

• A2M
• alpha 2Macroglobulin
• alpha 2-Macroglobulin
• alpha-2-M
• alpha-2-macroglobulin
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• CPAMD5
• CPAMD5DKFZp779B086
• FWP007
• S863-7

Background

alpha ₂-Macroglobulin (alpha 2 macroglobulin; also A2M and MAM) is a 185 kDa member of the protease inhibitor I39 family of proteins. It is secreted by the liver, circulates at a constant level in the blood, binds multiple growth factors, inhibits a wide variety of proteinases via steric trapping, is cleared by cell surface receptors and likely exists as a tetramer. Mature mouse A2M is 1471 amino acids (aa) in length. It is cleaved between Arg1239 and Asp1240 to generate two subunits, one that is 165 kDa in size and 1215 aa in length and a second that is 35 kDa in size and 256 aa in length. The protease inhibitor region (aa 686-744) is on the large subunit, while the receptor binding segment (aa 1395-1482) is on the smaller subunit. A2M has a counterpart gene termed A2M-P that is associated with pregnancy. The A2M-P molecule shares 56% aa identity with mouse A2M. Notably, mouse A2M-P is closer in aa sequence to human A2M (72%) and rat A2M (91%) than mouse A2M is to human A2M (59%) and rat A2M (56%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha 2-Macroglobulin Antibody (AF5798)(1)

Publication using AF5798

• Mediha Becirovic-Agic, Upendra Chalise, Mira Jung, Jocelyn R. Rodriguez-Paar, Shelby R. Konfrst, Elizabeth R. Flynn et al. Faster skin wound healing predicts survival after myocardial infarction American Journal of Physiology-Heart and Circulatory Physiology 2022-04-01 [PMID: 35089808] (Western Blot, Human)

Bioinformatics

• Gene Symbol: A2M