alpha 2-Macroglobulin Antibody (257316) [Unconjugated]

Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a2-Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and human serum, loaded at 0.5 mg/ml and 1:10 respectively. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/ml of Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 257316
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha 2-Macroglobulin Antibody (257316) [Unconjugated] Summary

• Immunogen: Human plasma-derived alpha ₂-Macroglobulin
• Specificity: Detects human alpha ₂-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with rhC5a or rmC5a.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: A2M
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 2-Macroglobulin Antibody (257316) [Unconjugated]

• A2M
• alpha 2Macroglobulin
• alpha 2-Macroglobulin
• alpha-2-M
• alpha-2-macroglobulin
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• CPAMD5
• CPAMD5DKFZp779B086
• FWP007
• S863-7

Background

Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha 2-Macroglobulin Antibody (MAB1938)(3)

Publications using MAB1938

• M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh Fecal host biomarkers predicting severity of Clostridioides difficile infection JCI Insight, 2021-01-11;0(0):. 2021-01-11 [PMID: 33232301] (Western Blot, Human)
• Telford G, Brown AP, Kind A, English JS, Pritchard DI Maggot chymotrypsin I from Lucilia sericata is resistant to endogenous wound protease inhibitors. Br. J. Dermatol., 2010-11-29;164(1):192-6. 2010-11-29 [PMID: 21175562] (Western Blot, Human)
• Jae-Sung Yu, Ben-Jiang Ma, Richard M. Scearce, Hua-Xin Liao, Barton F. Haynes Anti-Ebola MAb 17A3 reacts with bovine and human alpha-2-macroglobulin proteins Journal of Virological Methods 2010-09-01 [PMID: 20447422]

Bioinformatics

• Gene Symbol: A2M