alpha 2-Macroglobulin Antibody (257316) [Unconjugated]

Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha 2‑Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 257316
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha 2-Macroglobulin Antibody (257316) [Unconjugated] Summary

• Immunogen: Human plasma-derived alpha ₂-Macroglobulin
• Specificity: Detects human alpha ₂-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with rhC5a or rmC5a.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: A2M

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • Simple Western 10 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 2-Macroglobulin Antibody (257316) [Unconjugated]

• A2M
• alpha 2Macroglobulin
• alpha 2-Macroglobulin
• alpha-2-M
• alpha-2-macroglobulin
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• CPAMD5
• CPAMD5DKFZp779B086
• FWP007
• S863-7

Background

Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha 2-Macroglobulin Antibody (MAB1938)(1)

Publication using MAB1938

• Telford G, Brown AP, Kind A, English JS, Pritchard DI Maggot chymotrypsin I from Lucilia sericata is resistant to endogenous wound protease inhibitors. Br. J. Dermatol., 2011;164(1):192-6. 2011 [PMID: 21175562] (Western Blot, Human)

Bioinformatics

• Gene Symbol: A2M