ALCAM/CD166 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ALCAM/CD166 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
  Trp28-Ala526
  Accession # AAB59499
• Specificity: Detects human ALCAM/CD166 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse ALCAM, recombinant human (rh) ICAM-2, rhICAM-3, rhE-Selectin, and rhP-Selectin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALCAM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human ALCAM/CD166 Antibody (Catalog # MAB6561)
  ELISA Detection: Human ALCAM/CD166 Biotinylated Antibody (Catalog # BAF656)
  Standard: Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 656-AL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody [Biotin]

• activated leucocyte cell adhesion molecule
• activated leukocyte cell adhesion moleculeFLJ38514
• ALCAM
• CD166 antigen
• CD166
• MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be up‑regulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALCAM/CD166 Antibody (BAF656)(2)

Publications using BAF656

• MIN YE, YAN-LEI DU, YU-QIANG NIE, ZHI-WEI ZHOU, JIE CAO, YING-FEI LI Overexpression of activated leukocute cell adhesion molecule in gastric cancer is associated with advanced stages and poor prognosis and miR-9 deregulation Molecular Medicine Reports 2015-03-01 [PMID: 25395097] (ELISA Detection)
• Kulasingam V, Zheng Y, Soosaipillai A, Leon AE, Gion M, Diamandis EP Activated leukocyte cell adhesion molecule: a novel biomarker for breast cancer. Int. J. Cancer, 2009-07-01;125(1):9-14. 2009-07-01 [PMID: 19322904] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: ALCAM
• Uniprot:
  • AAB59499()