MCAM/CD146 Antibody (128018) [Unconjugated]

Western blot shows lysate of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCAM/CD146 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

MCAM/CD146 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

MCAM and PECAM are both involved in PNA-mediated cytokine secretion. The presence of ASF or neutralizing antibodies to MCAM or PECAM reduced PNA-mediated secretion of MCP-1 and IL-6 (A) from HUVECs. siRNA suppression of MCAM and PECAM expression (B) each abolished PNA-induced secretion of MCP-1 and IL-6 (C). Data are presented as Mean ± SD of three independent experiments, each in triplicate. **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34223877), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 128018
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MCAM/CD146 Antibody (128018) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MCAM
  Val24-Gly559
  Accession # AAA20824
• Specificity: Detects human MCAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm) OCAM, or rmMAdCAM-1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCAM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCAM/CD146 Antibody (128018) [Unconjugated]

• CD146 antigen
• CD146
• cell surface glycoprotein MUC18
• Cell surface glycoprotein P1H12
• L-Gicerin
• MCAM
• melanoma adhesion molecule
• melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
• Melanoma-associated antigen A32
• Melanoma-associated antigen MUC18
• MUC18
• MUC18Gicerin

Background

MCAM, also known as MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCAM/CD146 Antibody (MAB932)(14)

Publications using MAB932

• N McNamee, LR de la Fuen, MJ Santos-Mar, L O'Driscoll Proteomics profiling identifies extracellular vesicles&#039; cargo associated with tumour cell induced platelet aggregation BMC Cancer, 2022-09-29;22(1):1023. 2022-09-29 [PMID: 36171564] (Western Blot, Human)
• Weikun Wang, Paulina Sindrewicz-Goral, Chen Chen, Carrie A Duckworth, David Mark Pritchard, Jonathan M Rhodes et al. Appearance of peanut agglutinin in the blood circulation after peanut ingestion promotes endothelial secretion of metastasis-promoting cytokines Carcinogenesis 8/19/2021 [PMID: 34223877]
• Ralph El Hallal, Nana Lyu, Yuling Wang Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells Molecules 1/22/2021 [PMID: 33499047]
• Runhua Feng, Yuling Wang, Vijaya Ramachandran, Qinhong Ma, Matthew M. May, Ming Li et al. Characterization of novel neutralizing mouse monoclonal antibody JM1-24-3 developed against MUC18 in metastatic melanoma Journal of Experimental & Clinical Cancer Research 12/5/2020 [PMID: 33278894]
• Runhua Feng, Yuling Wang, Vijaya Ramachandran, Qinhong Ma, Matthew M. May, Ming Li et al. Characterization of novel neutralizing mouse monoclonal antibody JM1-24-3 developed against MUC18 in metastatic melanoma Journal of Experimental & Clinical Cancer Research 2020-12-05 [PMID: 33278894] (Human)
• J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv, 2020-02-26;6(9):eaax3223. 2020-02-26 [PMID: 32133394] (Flow Cytometry, Human)
• Stefanie Spiegler, Matthias Rath, Christiane D. Much, Barbara S. Sendtner, Ute Felbor Precise CCM1 gene correction and inactivation in patient‐derived endothelial cells: Modeling Knudson's two‐hit hypothesis in vitro Molecular Genetics & Genomic Medicine 7/1/2019 [PMID: 31124307]
• MP Zaborowski, K Lee, YJ Na, A Sammarco, X Zhang, M Iwanicki, PS Cheah, HY Lin, M Zinter, CY Chou, G Fulci, BA Tannous, CP Lai, MJ Birrer, R Weissleder, H Lee, XO Breakefiel Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles Cell Rep, 2019-04-02;27(1):255-268.e6. 2019-04-02 [PMID: 30943406] (Flow Cytometry, Human)
• SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration Mol Med Rep, 2019-02-01;0(0):. 2019-02-01 [PMID: 30720108] (Flow Cytometry, Rat)
• J Wang, W Anderson, J Li, LL Lin, Y Wang, M Trau A high-resolution study of in situ surface-enhanced Raman scattering nanotag behavior in biological systems J Colloid Interface Sci, 2018-11-12;537(0):536-546. 2018-11-12 [PMID: 30469121] (Dynamic Light Scattering, Human)
• Costa D, Vene R, Benelli R et al. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts Front Immunol 5/29/2018 [PMID: 29910806]
• Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke Stem Cells Int, 2016-10-20;2016(0):6104780. 2016-10-20 [PMID: 27840648] (Flow Cytometry, Human)
• Brun J, Abruzzese T, Rolauffs B, Aicher W, Hart M Choice of xenogenic-free expansion media significantly influences the myogenic differentiation potential of human bone marrow-derived mesenchymal stromal cells. Cytotherapy, 2016-03-01;18(3):344-59. 2016-03-01 [PMID: 26857228] (Flow Cytometry, Human)
• Im , Hyungsoo, Shao , Huilin, Park , Yong Il, Peterson , Vanessa, Castro , Cesar M, Weissleder , Ralph, Lee , Hakho Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor. Nat Biotechnol, 2014-04-20;32(5):490-5. 2014-04-20 [PMID: 24752081] (Antibody Profiling)

Bioinformatics

• Gene Symbol: MCAM
• Uniprot:
  • AAA20824()