ALCAM Products By Gene Name

HAF017). A specific band was detected for ALCAM/CD166 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for ALCAM/CD166 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse brain (cerebellumn) tissue, rat brain (cerebellum) tissue, H4-II-E-C3 rat hepatoma cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ALCAM/CD166 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0107) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C)." class="big_lightbox"> F0107) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C)." class="big_thumb" />

AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox"> AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

656-AL) was serially diluted and captured by Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 656-AL) was serially diluted and captured by Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

656-AL) was serially diluted and captured by Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 656-AL) was serially diluted and captured by Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

656-AL) was serially diluted and captured by Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 656-AL) was serially diluted and captured by Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 656-AL) was serially diluted and captured by Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

F0105B) or (B) unstained splenocytes . View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0105B) or (B) unstained splenocytes . View our protocol for Staining Membrane-associated Proteins. " alt="Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse ALCAM/CD166 Monoclonal Antibody (Catalog # mab1172) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) or (B) unstained splenocytes . View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

HAF007). A specific band was detected for ALCAM/CD166 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. For best results, non-reducing conditions only are recommended." class="big_lightbox"> HAF007). A specific band was detected for ALCAM/CD166 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. For best results, non-reducing conditions only are recommended." alt="Western blot shows lysates of HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ALCAM/CD166 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. For best results, non-reducing conditions only are recommended." class="big_thumb" />